大阪大学生物工学国際交流センター 藤山研究室  
研究概要業績研究室行事メンバー進路リンク アクセス ホームEnglish研究に興味のある方へ 藤山研通信

業績

【2024年4月1日更新】

::最近5年間の発表論文::

PubMedに記載されている論文についてはPubMedIDにリンクしています。

2023年

Tepjanta P, Fujiyama K, Misaki R and Kimkong I.
The N-linked glycosylation modifications in the hepatitis B surface protein impact cellular autophagy, HBV replication, and HBV secretion
PLoS One, 2024 Mar 15;19(3):e0299403.

Yamamoto Y, Kajiura H, Fukuchi H, Nishibu T, Fujiyama K and Kitagawa T.
Reconstitution of (1→3)-β-D-glucans measurement system using recombinant Limulus polyphemus Factor G
Appl Microbiol Biotechnol., 2023 Oct 26 doi: 10.1007/s00253-023-12808-6.

藤山和仁
糖鎖修飾改変植物細胞による医療用タンパク質生産
アグリバイオ 7(9)10‐14(2023)

Nguyen KD, Kajiura H, Kamiya R, Yoshida T, Misaki R, Fujiyama K.
Production and N-glycan engineering of Varlilumab in Nicotiana benthamiana.
Front Plant Sci., 2023 Aug 14 1215580

Wu CC, Honda K and Fujiyama K
Current advances in alteration of fatty acid profile in Rhodotorula toruloides: a mini-review
World J Microbiol. Biotechnol., 2023 Jun 39(9):234

Sato K, Yumioka H, Isoyama J, Dohi K, Yamanaka A, Ohashi T, Misaki R and Fujiyama K
High accumulation of the Man5GlcNAc2 structure by combining N-acetylglucosaminyltransferase I gene suppression and mannosidase I gene overexpression in Nicotiana tabacum SR1
J Biosci. Bioeng., 2023 In Press, Corrected Proof, Available online 11 June 2023

Choa JB, Sasaki T, Kajiura H, Ikuta K, Fujiyama K and Misaki R
Effects of various disaccharide adaptations on recombinant IgA1 production in CHO-K1 suspension cells
Cytotech., 2023 Jun 75(3):219-229.

::学会発表 2023年度:

 

2022年

藤山和仁
バイオプロセスを用いた有用物質生産技術
植物組織からの医療用タンパク質の抽出と精製、第7章、8節 (2022)

Kajiura H, Tatematsu K, Nomura T, Miyazawa M, Usami A, Tamura T, Sezutsu H and Fujiyama K
Insights into the quality of recombinant proteins produced by two different Bombyx mori expression systems
Sci. Rep., 2022 Nov 12(1):18502.

Macauyag EA, Kajiura H, Ohashi T, Misaki R and Fujiyama K
High-level transient production of a protease-resistant mutant form of human basic fibroblast growth factor in Nicotiana benthamiana leaves
Plant Biotech., 2022 39(3):291-301

Yamamoto Y, Kajiura H, Nishibu T and Fujiyama K
Mamestra brassicae NIAS-Mb-32 cell strain 2g2 enables high-yield recombinant protein production in baculovirus-free and baculovirus-based insect cell expression
J Biosci. Bioeng., 2022 Nov 134(5):432-440

Dent M, Mayer KL, Garcia NV, Guo H, Kajiura H, Fujiyama K and Matoba N
Impact of glycoengineering and anti-drug antibodies on the anti-cancer activity of a plant-made lectin-Fc fusion protein
Plant Biotechnol J., 2022 Jul 28

Kurniati A, Puspaningsih NNT, Putri KDA, Damayanti M, Purwani NN, Rahmah SA, Purkan, Fujiyama K, Sakka M, Sakka K, Kimura T, Rohman A, Baktir A and Sanjayaadg RE
Heterologous fusion gene expression and characterization of a novel carbohydrate binding module (Cbm36) to laccase (Lcc2)
Biocatal. Atricul. Biotech., 2022 Jul 42:102377.

Kajiura H, Eguchi T, Uchino K, Tatematsu K, Tamura T and Sezutsu H
Temporal analysis of N-acetylglucosamine extension of N-glycans in the middle silk gland of silkworm Bombyx mori
J Biosci. Bioeng., 2022 Jun 133(6):533-540.

Kajiura H, Hiwasa-Tanase K, Ezura H, Fujiyama K
Effect of fruit maturation on N-glycosylation of plant-derived native and recombinant miraculin
Plant Physiol Biochem., 2022 May 178:70-79

Uthailak N, Kajiura H, Misaki R, Fujiyama K
Production of recombinant β-glucocerebrosidase in wild-type and glycoengineered transgenic Nicotiana benthamiana root cultures with different N-glycan profiles.
J Biosci. Bioeng., 2022 May 133(5):481-488

::学会発表 2022年度:

 

2021年

Misaki R, Iwasaki M, Takechi H, Yamano-Adachi N, Ohashi T, Kajiura H, Fujiyama K
Establishment of serum-free adapted Chinese hamster ovary cells with double knockout of GDP-mannose-4,6-dehydratase and GDP-fucose transporter.
Cytotechnology, 2022 Feb 74(1):163-179.

Uthailak N, Kajiura H, Misaki R, Fujiyama K
Production of recombinant b-glucocerebrosidase in wild-type and glycoengineered transgenic Nicotiana benthamiana root cultures with different N-glycan profiles
J Biosci. Bioeng., 2022 Feb 18;S1389-1723(22)00002-0.

Ohashi T, Sari N, Misaki R, Fujiyama K.
Biochemical characterization of Arabidopsis clade F polygalacturonase shows a substrate preference toward oligogalacturonic acids.
J Biosci. Bioeng. 2022 Jan 133(1):1-7

藤山和仁
植物を用いた医療用タンパク質生産
Applied Cell Biology Japan 2021 34:17-32

Sariyatun R, Kajiura H, Limkul J, Misaki R, Fujiyama K.
Analysis of N-glycan profile of Arabidopsis alg3 cell culture.
Plant Biotechnol (Tokyo)., 2021 Dec 25;38(4):463-467

Chan JLR. , Leon MP. , Quimio JMUP., Fujiyama K, Sabino NG., and Mendoza BC.
The Potential Bacteriocinogenic Activity of Putative Bacteriocins of E. coli from Bat Guano against Enterohemorrhagic E. coli Strains
Philippine J Veterinary Med., 2021 58 (2):167-181

Yamamoto C, Ogura M, Uemura R, Megumi M, Kajiura H, Misaki R, Fujiyama K, Kimura Y.
Improved assay system for acidic peptide: N-glycanase (aPNGase) activity in plant extracts.
Anal. Biochem. 2021 Sep 9:114367. doi: 10.1016/j.ab.2021.114367.

Sariyatun R, Florence, Kajiura H, Ohashi T, Misaki R, Fujiyama K.
Production of Human Acid-Alpha Glucosidase With a Paucimannose Structure by Glycoengineered Arabidopsis Cell Culture.
Front Plant Sci., 2021 Jul 14;12:703020.

藤山和仁
植物を用いたバイオリアクターによるワクチン、抗体の生産
PHARM STAGE 21(4): 18-22 2021年7月号

Uthailak N, Kajiura K, Misaki R, Fujiyama K
Transient Production of Human β-Glucocerebrosidase with Mannosidic-Type N-Glycan Structure in Glycoengineered Nicotiana benthamiana Plants
Front Plant Sci., 2021 Jun 7;12:683762.

Wu CC, Ohashi T, Kajiura H, Sato Y, Misaki R, Honda K, Limtong S, Fujiyama K
Functional characterization and overexpression of Δ12-desaturase in the oleaginous yeast Rhodotorula toruloides for production of linoleic acid-rich lipids.
J Biosci Bioeng., 2021 Jun 131(6):631-639.

Shirai S, Uemura R, Maeda M, Kajiura H, Misaki R, Fujiyama K, Kimura Y.
Direct evidence of cytosolic PNGase activity in Arabidopsis thaliana: in vitro assay system for plant cPNGase activity.
Biosci Biotechnol Biochem., 2021 May 25;85(6):1460-1463.

Kajiura H, Miyauchi R, Kakudo A, Ohashi T, Misaki R, Fujiyama K
Bombyx mori β1,4-N-acetylgalactosaminyltransferase possesses relaxed donor substrate specificity in N-glycan synthesis
Scientific Reports, 2021 Mar 9;11(1):5505.

Kajiura H, Yoshizawa T, Tokumoto Y, Suzuki N, Takeno S, Jumtee-Takeno K, Yamashita T, Tanaka S, Kaneko Y, Fujiyama K, Matsumura H, Nakazawa Y
Structure-function studies of ultrahigh molecular weight isoprenes provide key insights into their biosynthesis
Commun Biol., 2021 Feb 16;4(1):215.

::学会発表 2021年度:

2020年

Gungupalli MP, Kajiura H, Ishimizu T, Bhattacharyya D
Alkaline hydrolysis of coconut pith: process optimization, enzymatic saccharification, and nitrobenzene oxidation of Kraft lignin
Biomass Conv. Bioref., 2020 https://doi.org/10.1007/s13399-020-00890-z

Wachananawat B, Kuroha T, Takenaka Y, Kajiura H, Naramoto S, Yokoyama R, Ishizaki K, Nishitani K, Ishimizu T.
Diversity of Pectin Rhamnogalacturonan I Rhamnosyltransferases in Glycosyltransferase Family 106
Front Plant Sci., 2020 Jul 2;11:997.

Arifin M, Budiman C, Fujiyama K, Arief II
Kinetic and Thermodynamic Study of Plantaricin IIA-1A5, a Bacteriocin Produced by Indonesian Probiotic Lactobacillus plantarum IIA-1A5
Protein Pept Lett., 2020 Nov doi: 10.2174/0929866527999201123213841.

Ohashi T, Fujisawa Y, Hayes MR, Misaki R, Pietruszka J, Fujiyama K
Transglycosylation toward naringenin-7-O-glucoside using an N180H mutant of Coprinopsis cinerea endo-β-N-acetylglucosaminidase
Biochem Biophys Res Commun., 2020 Sep 10; 530(1): 155-159.

Kajiura H, Nakamura Y, Nishimura M, Ohashi T, Misaki R, Fujiyama K
Characterization of Bombyx mori N-acetylglucosaminyltransferase II splicing variants
Biochem Biophys Res Commun., 2020 Aug 20;529 (2): 404-410.

Wongwisansri S, Promdonkoy P, Likhitrattanapisal S, Harnpichanchai P, Fujiyama K, Kaneko Y, Eurwilaichitr L, Ingsrisawang S, Tanapongpipat S
Mating-type switching and mating-type gene array expression in the methylotrophic yeast Ogataea thermomethanolica TBRC656
Microbiological Resarch, 2020 Feb 232:126372

Matsumura T, Amatsu S, Misaki R, Yutani M, Anariwa D, Kohda T, Fujiyama K, Ikuta K, Fujinaga Y
Fully Human Monoclonal Antibodies Effectively Neutralizing Botulinum Neurotoxin Serotype B
Toxins, 2020 May 7;12(5):302.

Nguyen TS, Misaki R, Ohashi T, Fujiyama K.
Enhancement of sialylation in rIgG in glyco-engineered Chinese hamster ovary cells.
Cytotechnology, 2020 Jun 72(3):343-355.

::学会発表 2020年度:

2019年

Fujiyama, K. (2019)
‘Plant and Glycan’, in Taniguchi, N., et al. (eds.) Glycoscience: Basic Science to Applications. Singapore: Springer,pp. 114-115.
Singare: Springer,

Matsuura H, Ujiie K, Duyen TTM, Izutsu K, Fujiyama K, Maeda S, Harada K, Hirata K.
Development of a Paper-Based Luminescence Bioassay for Therapeutic Monitoring of Aminoglycosides: a Proof-of-Concept Study.
Appl Biochem Biotechnol., 2019 Nov 189(3):798-809.

Bunsangiam S, Sakpuntoon V, Srisuk N, Ohashi T, Fujiyama K & Limtong S
Biosynthetic Pathway of Indole-3-Acetic Acid in Basidiomycetous Yeast Rhodosporidiobolus fluvialis
Mycobiology, 2019 Jul 15;47(3):292-300.

Flores RJD, Ohashi T, Sakai K, Gonoi T, Kawasaki H, Fujiyama K.
The neutral N-linked glycans of the Basidiomycetous yeasts Pseudozyma antarctica and Malassezia furfur (Subphylum Ustilaginomycotina).
J Gen Appl Microbiol., 2019 May 21;65(2):53-63.

Tsai YY, Ohashi T, Wu CC, Bataa D, Misaki R, Limtong S, Fujiyama K.
Delta-9 fatty acid desaturase overexpression enhanced lipid production and oleic acid content in Rhodosporidium toruloides for preferable yeast lipid production.
J Biosci Bioeng., 2019 Apr 127(4):430-440.

Ohashi H, Ohashi T, Misaki R, Fujiyama K.
Arabidopsis thaliana α1,2-l-fucosyltransferase catalyzes the transfer of l-galactose to xyloglucan oligosaccharides.
FEBS Lett., 2019 Jan 593(2):187-194.

Arofatullah NA, Kabirun S, Fujiyama K, Widianto D
Molecular identification and in vitro propagation of arbuscular mycorrhiza from tea plant rhizosphere
Current Research in Environmental & Applied Mycology 2019 9(1): 92–102
*本論文に示されている成果の一部は、Arofatullah NAがBiotechnology School in Asia programに在籍中になされてたものである

::学会発表 2019年度:

::2018年以前の発表論文::

Copyright (C) Fujiyama lab., ICBiotech, Osaka University