2019年

Fujiyama, K. (2019)
‘Plant and Glycan’, in Taniguchi, N., et al. (eds.) Glycoscience: Basic Science to Applications. Singapore: Springer,pp. 114-115.
Singare: Springer,

Matsuura H, Ujiie K, Duyen TTM, Izutsu K, Fujiyama K, Maeda S, Harada K, Hirata K.
Development of a Paper-Based Luminescence Bioassay for Therapeutic Monitoring of Aminoglycosides: a Proof-of-Concept Study.
Appl Biochem Biotechnol., 2019 Nov 189(3):798-809.

Bunsangiam S, Sakpuntoon V, Srisuk N, Ohashi T, Fujiyama K & Limtong S
Biosynthetic Pathway of Indole-3-Acetic Acid in Basidiomycetous Yeast Rhodosporidiobolus fluvialis
Mycobiology, 2019 Jul 15;47(3):292-300.

Flores RJD, Ohashi T, Sakai K, Gonoi T, Kawasaki H, Fujiyama K.
The neutral N-linked glycans of the Basidiomycetous yeasts Pseudozyma antarctica and Malassezia furfur (Subphylum Ustilaginomycotina).
J Gen Appl Microbiol., 2019 May 21;65(2):53-63.

Tsai YY, Ohashi T, Wu CC, Bataa D, Misaki R, Limtong S, Fujiyama K.
Delta-9 fatty acid desaturase overexpression enhanced lipid production and oleic acid content in Rhodosporidium toruloides for preferable yeast lipid production.
J Biosci Bioeng., 2019 Apr 127(4):430-440.

Ohashi H, Ohashi T, Misaki R, Fujiyama K.
Arabidopsis thaliana α1,2-l-fucosyltransferase catalyzes the transfer of l-galactose to xyloglucan oligosaccharides.
FEBS Lett., 2019 Jan 593(2):187-194.

Arofatullah NA, Kabirun S, Fujiyama K, Widianto D
Molecular identification and in vitro propagation of arbuscular mycorrhiza from tea plant rhizosphere
Current Research in Environmental & Applied Mycology 2019 9(1): 92–102
＊本論文に示されている成果の一部は、Arofatullah NAがBiotechnology School in Asia programに在籍中になされてたものである

::学会発表 2019年度:

2018年

Fujiyama, K. (2018)
‘Glycoprotein Biosynthesis of Silkworm’, in Maenaka, K. and Park, E.Y. (eds.) Silkworm Biofactory. Boca Raton: CRC Press, pp. 107-126.

Nakamura M, Tran ML, Le QL, Suzuki H, Nguyen CM, Vu HG, Tamura K, Nguyen VT, Suzuki H, Misaki R, Muranaka T, Ninh KB, Fujiyama K, Seki H.
Transcriptome sequencing and identification of cytochrome P450 monooxygenases involved in the biosynthesis of maslinic acid and corosolic acid in Avicennia marina
Plant Biotechnology, 2018 Dec 25; 35(4): 341–348.

藤山和仁
カイコの高次な生物機能の“革命的”利用、研究ノート
生産と技術、第70巻、第4号、65-8 (2018)

Wu CC, Tsai YY, Ohashi T, Misaki R, Limtong S, Fujiyama K.
Isolation of a thermotolerant Rhodosporidium toruloides DMKU3-TK16 mutant and its fatty acid profile at high temperature.
FEMS Microbiol Lett., 2018 Nov 365(21).

Priyambada SA, Misaki R, Okamoto T, Ohashi T, Ueda K, Matsuura Y, Fujiyama K.
St6gal1 knockdown alters HBV life cycle in HepAD38 cells.
Biochem Biophys Res Commun., 2018 Sep 10; 503(3): 1841-184.

Naphatsamon U, Ohashi T, Misaki R, Fujiyama K.
The Production of Human β-Glucocerebrosidase in Nicotiana benthamiana Root Culture.
Int J Mol Sci., 2018 Jul 6; 19(7)

大橋貴生
機能性フラボノイド配糖体の醗酵生産、研究ノート
生産と技術、第70巻、第1号、103-5 (2018)

Suganuma M, Nomura T, Higa Y, Kataoka Y, Hunaguma S, Okazaki H, Suzuki T, Fujiyama K, Sezutsu H, Tatematsu K, Tamura T.
N-glycan sialylation in a silkworm-baculovirus expression system
J Biosci Bioeng., 2018 Jul 126(1): 9-14.

Nguyen TS, Misaki R, Ohashi T, Fujiyama K.
Enhancement of glycosylation by stable co-expression of two sialylation related enzymes on Chinese hamster ovary cells
J Biosci Bioeng., 2018 Jul 126(1): 102-110.

Morokuma D, Hino M, Masuda A, Mon H, Fujiyama K, Kajiura H, Kusakabe T, Lee JM,
A truncated form of human alpha 1-acid glycoprotein is useful as a molecular tool for insect glycobiology
Int J Indust. Entomol., 2018 36(1): 15-24.

Roychowdhury S, Youngjun Oh, Kajiura H, Hamorsky K, Fujiyama K, Matoba N.
Hydroponic treatment of Nicotiana benthamiana with kifunensine modifies the N-glycans of recombinant glycoprotein antigens to predominantly Man9 high-mannose type upon transient overexpression
Front. Plant Sci., 2018 Jan 30; 9: 62

Kajiura H,Hiwasa-Tanase K,Ezura H, Fujiyama K
Comparison of N-glycosylation on recombinant miraculin expressed in tomato plants with native miraculin
Plant Biotechnology, 2018 Vol. 35 No. 4 pp. 375-379.

Polburee P, Ohashi T, Tsai YY, Sumyai T, Lertwattanasakul N, Limtong S, Fujiyama K.
Molecular cloning and overexpression of DGA1, an acyl-CoAdependent diacylglycerol acyltransferase, in the oleaginous yeast Rhodosporidiobolus fluvialis DMKU-RK253
Microbiology, 2018 Jan 164(1): 1-10.

Ishinishi R, Matsuura H, Tanaka S, Nozawa S, Tanada K, Kawashita N, Fujiyama K, Miyasaka H, Hirata K.
Isolation and characterization of a stress-responsive gene encoding a CHRD domain-containing protein from a halotolerant green alga.
Gene, 2018 Jan 15; 640:14-20.

::学会発表 2018年度:

2017年

大橋貴生
高等植物のペクチン生合成および分解に関与する糖質関連酵素群、今日の話題
化学と生物、第55巻、第7号、454-6 (2017)

大橋貴生
酵母ベクターの種類と歴史、続・生物工学基礎講座 バイオよもやま話
生物工学会誌、第95巻、第1号、16-9 (2017)

Pierce OM, McNair GR, He X, Kajiura H, Fujiyama K, Kermode AR.
N-glycan structures and downstream mannose-phosphorylation of plant recombinant human alpha-L-iduronidase: toward development of enzyme replacement therapy for mucopolysaccharidosis I.
Plant Mol Biol., 2017 Dec 95(6): 593-606.

Ohashi H, Wahl C, Ohashi T, Elling L and Fujiyama K.
Effective synthesis of guanosine 5'-diphospho-β-L-galactose using bacterial L-fucokinase/guanosine 5'-diphosphate-L-fucose pyrophosphorylase
Advanced Synthesis & Catalysis, 2017 Dec 359(23):4227-4234. 

Priyambada SA, Misaki R, Okamoto T, Okamoto Y, Ohashi T, Ueda K, Matsuura Y, Fujiyama K.
Cell surface N-glycan alteration in HepAD38 cell lines expressing Hepatitis B virus.
Virus Res., 2017 Jun 20; 238: 101-109.

Kajiura H, Suzuki N, Tokumoto Y, Yoshizawa T, Takeno S, Fujiyama K, Kaneko Y, Matsumura H, Nakazawa Y.
Two Eucommia farnesyl diphosphate synthases exhibit distinct enzymatic properties leading to end product preferences.
Biochimie, 2017 Aug 139: 95-106.

Flores RJ, Ohashi T, Kawasaki H, Fujiyama K.
The Neutral N-linked Glycans of the Ustilaginomycete Yeast Sympodiomycopsis paphiopedili.
Yeast, 2017 Jul 34(7): 305-317.

Ohashi T, Jinno J, Inoue Y, Ito S, Fujiyama K, Ishimizu T.
A polygalacturonase localized in the Golgi apparatus in Pisum sativum.
J Biochem, 2017 Sep 1;162(3):193-201.
*立命館大　石水先生等との共同研究

Tsai YY, Ohashi T, Kanazawa T, Polburee P, Misaki R, Limtong S, Fujiyama K.
Development of a sufficient and effective procedure for transformation of an oleaginous yeast, Rhodosporidium toruloides DMKU3-TK16
Curr Genet., 2017 May 63(2): 359-371.
*タイ・カセサート大との共同研究

Uehara Y, Tamura S, Maki Y, Yagyu K, Mizoguchi T, Tamiaki H, Imai T, Ishii T, Ohashi T, Fujiyama K, Ishimizu T.
Biochemical characterization of rhamnosyltransferase involved in biosynthesis of pectic rhamnogalacturonan I in plant cell wall.
Biochem Biophys Res Commun., 2017 Apr 22;486(1):130-136.
*立命館大　石水先生等との共同研究

Ohashi H, Ohashi T, Kajiura H, Misaki R, Kitamura S, Fujiyama K.
Fucosyltransferases produce N-glycans containing core L-galactose.
Biochem Biophys Res Commun., 2017 Jan 29;483(1):658-663.
*大阪府立大　北村先生との共同研究

::学会発表 2017年度:

2016年

De Martinis D, Rybicki EP, Fujiyama K, Franconi R, Benvenuto E.
Editorial: Plant Molecular Farming: Fast, Scalable, Cheap, Sustainable.
Front Plant Sci., 2016 Aug 3;7:1148.

Misaki R, Fukura N, Kajiura H, Yasugi M, Kubota-Koketsu R, Sasaki T, Momota M,Ono K, Ohashi T, Ikuta K, Fujiyama K.
Recombinant production and characterization of human anti-influenza virus monoclonal antibodies identified from hybridomas fused with human lymphocytes.
Biologicals., 2016 44(5); 394-402
*SATRERSでの研究成果

Takamatsu S, Shimomura M, Kamada Y, Maeda H, Sobajima T, Hikita H, Iijima M, Okamoto Y, Misaki R, Fujiyama K, Nagamori S, Kanai Y, Takehara T, Ueda K, Kuroda S, Miyoshi E.
Core-fucosylation plays a pivotal role in hepatitis B pseudo virus infection: a possible implication for HBV glyco-therapy.
Glycobiology., 2016 Nov 26(11):1180-1189. 
*医学部、産研との共同研究（厚労科研）

Pirapan Polbureea P, Yongmanitchaia W, Honda K, Ohashi T, Yoshida T, Fujiyama K, Limtonga S
Lipid production from biodiesel-derived crude glycerol by Rhodosporidium fluviale DMKU-RK253 using temperature shift with high cell density
Biochem　Eng J., 2016 112, 208-218
*タイ・カセサート大との共同研究

Limkul J, Iizuka S, Sato Y, Misaki R, Ohashi T, Ohashi T, Fujiyama K.
The production of human glucocerebrosidase in glyco-engineered Nicotiana benthamiana plants.
Plant Biotechnol J., 2016 14(8); 1682-1694
*慈恵医大・大橋十也教授グループとの共同研究

Trempel F, Kajiura H, Ranf S, Grimmer J, Westphal L, Zipfel C, Scheel D, Fujiyama K and Lee J
Altered glycosylation of exported proteins, including surface immune receptors, compromises calcium and downstream signaling responses to microbe-associated molecular patterns in Arabidopsis thaliana.
BMC Plant Biol., 2016 Jan 28;16:31.
*ドイツLeibniz Institute of Plant Biochemistry（Halle/Saale）等との共同研究

Roongsawang N, Puseenam A, Kitikhun S, Sae-Tang K, Harnpicharnchai P, Ohashi T, Fujiyama K, Tirasophon W, Tanapongpipat S.
A Novel Potential Signal Peptide Sequence and Overexpression of ER-Resident Chaperones Enhance Heterologous Protein Secretion in Thermotolerant Methylotrophic Yeast Ogataea thermomethanolica.
Appl Microbiol Biotechnol., 2016 178(4); 710-724.
　 　*タイ・国立研究所BIOTECとの共同研究

::学会発表 2016年度:

2015年

Ohashi T, Hasegawa Y, Misaki R, Fujiyama K.
Substrate preference of citrus naringenin rhamnosyltransferases and their application to flavonoid glycoside production in fission yeast.
Appl Microbiol Biotechnol., 2015 100(2):687-696.

Polburee P, Yongmanitchai W, Lertwattanasakul N, Ohashi T, Fujiyama K, Limtong S.
Characterization of oleaginous yeasts accumulating high levels of lipid when cultivated in glycerol and their potential for lipid production from biodiesel-derived crude glycerol
Fungal Biol., 2015 119(12):1194-1204.
　　*タイ・カセサート大との共同研究

Limkul J, Misaki R, Kato K, Fujiyama K.
The combination of plant translational enhancers and terminator increase the expression of human glucocerebrosidase in Nicotiana benthamiana plants
Plant Science, 2015 240: 41-49.

Kajiura H, Hamaguchi Y, Mizushima H, Misaki R, Fujiyama K.
Sialylation potentials of the silkworm, Bombyx mori; B. mori possesses an active α2,6-sialyltransferase.
Glycobiology, 2015 25(12): 1441-1453.

Arief II, Jenie BSL, Astawan M, Fujiyama K, Witarto AB.
Identification and probiotic characteristics of lactic acid bacteria isolated from Indonesian local beef
Asian J Animal Sciences, 2015 9 (1): 25-36.
　　*インドネシア・ボコール農大との共同研究

藤山和仁
「第4編　第3章 糖鎖の合成－生物系　第3節　植物を利用した糖タンパク質合成」
糖鎖の新機能開発・応用ハンドブック　監修：秋吉一成
株式会社 ＮＴＳ, 2015年8月

H. Kajiura, K. Fujiyama
Im“plant”ing of Mammalian Glycosyltransferase Gene into Plant Suspension-Cultured Cells Using Agrobacterium-Mediated Transformation
Methods Mol Biol., 2015 1321: 225-232.

Hamorsky KT, Kouokam JC, Jurkiewicz JM, Nelson B, Moore LJ, Husk AS, Kajiura H, Fujiyama K, Matoba N.
N-glycosylation of cholera toxin B subunit in Nicotiana benthamiana: impacts on host stress response, production yield and vaccine potential.
Sci Rep., 2015 5:8003.

Srichaisupakit A, Ohashi T, Misaki R, Fujiyama K.
Production of the Initial-Stage Eukaryotic N-Glycan and Protein Glycosylation in Escherichia coli.
J Biosci Bioeng., 2015 119(4): 399-405.

Nomura T, Suganuma M, Higa Y, Kataoka Y, Funaguma S, Okazaki H, Suzuki T, Kobayashi I, Sezutsu H, Fujiyama K.
Improvement of glycosylation structure by suppression of β-N-acetylglucosaminidases in silkworm.
J Biosci Bioeng., 2015 119(2): 131-136.

::学会発表 2015年度:

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2014年

Fujiyama K.
Heterologous Glycoprotein Production (Plant)
In: Glycoscience: Biology and Medicine. Naoyuki Taniguchi,Tamao Endo, Gerald W. Hart, Peter H. Seeberger, Chi-Huey Wong (Ed.) Springer Pages 1545-1549 ISBN: 978-4-431-54840-9 (Print) 978-4-431-54841-6 (Online)., 2014 Mar

梶浦裕之, 藤山和仁
植物N-結合型糖鎖修飾酵素の異種細胞における発現と修飾経路の考察」
化学と生物 Vol.52 No.11 719-724　(2014)

Polburee P, Yongmanitchai W, Ohashi T, Fujiyama K, Limtong S.
Barnettozyma siamensis f.a., sp. nov., a novel lipid accumulating ascomycete yeast species isolated in Thailand.
Int J Syst Evol Microbiol., 2014 64(Pt 9): 3053-3057.

Yumioka-Ito H, Misaki R, Yokoro M, Suzuki M, Yamashita H, Hiemori-Kondo M, Kimoto M, Kato K, Fujiyama K, Tsuji H.
Cloning of a cDNA encoding the Gly m Bd 28K precursor and its vacuole transport in tobacco BY2 suspension-cultured cells.
J Nutr Sci Vitaminol., 2014 60(2): 129-139.

Pitaksajjakul P, Benjathummarak S, Pipattanaboon C, Wongwit W, Okabayashi T, Kuhara M, Misaki R, Fujiyama K, Ramasoota P.
Antibody Germline Characterization of Cross-neutralizing human IgGs against 4 serotypes of Dengue virus.
Biochem Biophys Res Commun., 2014 446(2): 475-80.

Srichaisupakit A, Ohashi T, Fujiyama K.
Identification of a protein glycosylation operon from Campylobacter jejuni JCM 2013 and its heterologous expression in Escherichia coli.
J Biosci Bioeng., 2014 118(3): 256-262.

::学会発表 2014年度:

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2013年

Yasugi M, Kubota-Koketsu R, Yamashita A, Kawashita N, Du A, Misaki R, Kuhara M, Boonsathorn N, Fujiyama K, Okuno Y, Nakaya T, Ikuta K.
Emerging Antigenic Variants at the Antigenic Site Sb in Pandemic A(H1N1)2009 Influenza Virus in Japan Detected by a Human Monoclonal Antibody.
PLoS One., 2013 8(10): e77892

Kajiura H, Wasai M, Kasahara S, Takaiwa F, Fujiyama K.
N-Glycosylation and N-glycan moieties of CTB expressed in rice seeds.
Mol Biotechnol., 2013 Jul;54(3): 784-794.

藤山和仁
「糖鎖プロセシング酵素、マンノシダーゼ」
応用糖質科学 2013年　第3巻　第1号 87-92.

Wang S, Takahashi H, Kajiura H, Kawakatsu T, Fujiyama K, Takaiwa F.
Transgenic Rice Seeds Accumulating Recombinant Hypoallergenic Birch Pollen Allergen Bet v 1 Generate Giant Protein Bodies.
Plant Cell Physiol., 2013 54(6): 917-933.

Sasaki T, Setthapramote C, Kurosu T, Nishimura M, Asai A, Omokoko MD, Pipattanaboon C, Pitaksajjakul P, Limkittikul K, Subchareon A, Chaichana P, Okabayashi T, Hirai I, Leuangwutiwong P, Misaki R, Fujiyama K, Ono K, Okuno Y, Ramasoota P, Ikuta K.
Dengue virus neutralization and antibody-dependent enhancement activities of human monoclonal antibodies derived from dengue patients at acute phase of secondary infection.
Antiviral Res., 2013 98(3): 423-431.

Hamorsky KT, Kouokam JC, Bennett LJ, Baldauf KJ, Kajiura H, Fujiyama K, Matoba N.
Rapid and scalable plant-based production of a cholera toxin B subunit variant to aid in mass vaccination against cholera outbreaks.
PLoS Negl Trop Dis., 2013 7(3): e2046  

University of Louisville School of Medicineとの共同研究です。Nicotiana benthamianaを使い、 cholera toxin B subunit の効率的生産に関して調査しました。

Yasugi M, Kubota-Koketsu R, Kawashita N, Anariwa D, Misaki R, Kuhara M, Boonsathorn N, Fujiyama K, Okuno S, Nakaya T, Ikuta K.
Human Monoclonal Antibodies Broadly Neutralizing against Influenza B Virus.
PLoS Pathog., 2013 9(2): e1003150

大阪大学微生物病研究所等との共同研究の成果です。
インフルエンザBウィルスに対するヒト由来の抗体について、その遺伝子構造・性状に関して明らかにしました。

Watanabe S, Kakudo A, Ohta M, Mita K, Fujiyama K, Inumaru S.
Molecular cloning and characterization of the alpha-glucosidase II from Bombyx mori and Spodoptera frugiperda.
Insect Biochem Mol Biol., 2013 43(4): 319-327.

（独）動物衛生研究所との共同研究の成果です。
カイコのエサであるクワにはグルコシダーゼ阻害物質が含まれているにも関わらず、カイコは問題なく摂取しています。カイコよりグルコシダーゼ遺伝子を単離し、阻害剤抵抗性を調査しました。

::学会発表 2013年度

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2012年

Kajiura H, Tanaka R, Kato K, Hamaguchi Y, Seki T, Fujiyama K.
N-Glycan Structures of glycoproteins in suspension-cultured Arabidopsis thaliana T87 cells.
Plant Biotechnol., 2012 29: 489-493.

Kajiura H, Wasai M, Kasahara S, Takaiwa F, Fujiyama K.
N-Glycosylation and N-glycan moieties of CTB expressed in rice seeds. Mol Biotechnol., 2012 54(3): 784-794.

藤山和仁
創立90周年記念特別企画　特集―バイオ技術10年の軌跡―
植物の潜在機能を活用するバイオテクノロジー
「植物を使った医療用タンパク質生産の挑戦」
生物工学会誌－第90巻9号 563-566

Ohashi T, Fujiyama K, Takegawa K.
Identification of novel α1,3-galactosyltransferase and elimination of
α-galactose-containing glycans by disruption of multiple
α-galactosyltransferase genes in Schizosaccharomyces pombe.
J Biol Chem., 2012 287(46): 38866-75.

Hong Z, Kajiura H, Su W, Jin H, Kimura A, Fujiyama K, Li J.
Evolutionarily conserved glycan signal to degrade aberrant brassinosteroid receptors in Arabidopsis.
Proc Natl Acad Sci USA., 2012 109(28): 11437-42.

梶浦裕之、三崎亮、藤山和仁
「第IV編　第2章　植物における糖鎖の制御」
バイオ医薬品製造の効率化と生産基材の開発　監修：山口照英
株式会社 シーエムシー出版, 2011年4月

Lombardi R, Donini M, Villani ME, Brunetti P, Fujiyama K, Kajiura H, Paul M, Ma J, Benvenuto E.
Production of different glycosylation variants of the tumour-targeting mAb H10 in Nicotiana benthamiana. Influence on expression yield and antibody degradation.
Transgenic Res., 2012 21(5): 1005-21.

Onitsuka M, Kim WD, Ozaki H, Kawaguchi A, Honda K, Kajiura H, Fujiyama K, Asano R, Kumagai I, Ohtake H, Omasa T.
Enhancement of sialylation on humanized IgG-like bispecific antibody by overexpression of α2,6-sialyltransferase derived from Chinese hamster ovary cells.
Appl Microbiol Biotechnol., 2012 94(1): 69-80.

Kajiura H, Okamoto T, Misaki R, Matsuura Y, Fujiyama K.
Arabidopsis β1,2-xylosyltransferase: Substrate specificity and participation in the plant-specific N-glycosylation pathway.
J Biosci Bioeng., 2012 113(1): 48-54.

::学会発表 2012年度:

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2011年

三崎亮、藤山和仁
第11章　植物生産系を利用した糖タンパク質合成技術
バイオ医薬品開発における糖鎖技術　監修：早川堯夫・掛樋一晃・平林 淳
株式会社 シーエムシー出版、2011年11月

R. Misaki, Y. Sakai, T. Omasa, K. Fujiyama, and T. Seki.
N-terminal vacuolar sorting signal at the mouse antibody alters the N-linked glycosylation pattern in suspension-cultured tobacco BY2 cells.
J Biosci Bioeng., 2011 112(5): 476-484.

Y. Kimura, Y. Takeoka, M. Inoue, M. Maeda, and K. Fujiyama.
Double-Knockout of Putative Endo-β-N-acetylglucosaminidase (ENGase) Genes in Arabidopsis thaliana: Loss of ENGase Activity Induced Accumulation of High-Mannose Type Free N-Glycans Bearing N,N'-Acetylchitobiosyl Unit.
Biosci Biotechnol Biochem., 2011 75(5): 1019-21.

H. Kaulfuerst-Soboll, S. Rips, H. Koiwa, H. Kajiura, K. Fujiyama, and A. von Schaewen.
Reduced immunogenicity of Arabidopsis hybrid glycosylation1 (hgl1) N-glycans due to altered accessibility of xylose and core fucose epitopes.
J Biol Chem., 2011 286(26): 22955-64.

J. Isoyama-Tanaka, K. Dohi, R. Misaki, and K. Fujiyama.
Improved expression and characterization of recombinant human Golgi α1,2-mannosidase I isoforms (IA2 and IC) by Escherichia coli.
J Biosci Bioeng., 2011 112(1): 14-9.

K. Dohi, J. Isoyama-Tanaka, R. Misaki, and K. Fujiyama.
Jack bean alpha-mannosidase digestion profile of hybrid-type N-glycans: effect of reaction pH on substrate preference.
Biochimie., 2011 93(4): 766-71.

H. Kajiura, R. Misaki, K. Fujiyama, and T. Seki.
Stable coexpression of two human sialylation enzymes in plant suspension-cultured tobacco cells.
J Biosci Bioeng., 2011 111(4): 471-7.

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2010年

R. Misaki, M. Morimatsu, T. Uemura, S. Waguri, E. Miyoshi, N. Taniguchi, M. Matsuda, and T. Taguchi.
Palmitoylated Ras proteins traffic through recycling endosomes to the plasma membrane during exocytosis.
J Cell Biol ., 2010 191(1): 23-9.

T. Nomura, M. Ikeda, S. Ishiyama, K. Mita, T. Tamura, T. Okada, K. Fujiyama, and A. Usami.
Cloning and characterization of a beta-N-acetylglucosaminidase (BmFDL) from silkworm Bombyx mori.
J Biosci Bioeng ., 2010 110(4): 386-91.

H. Kajiura, T. Seki, and K. Fujiyama.
Arabidopsis thaliana ALG3 mutant synthesizes immature oligosaccharides in the ER and accumulates unique N-glycans.
Glycobiology., 2010 20(6): 736-751.

Asparagine-linked glycosylation 3 (ALG3)はER膜内腔での糖鎖修飾の第一段階を担う酵素で，細胞質側で合成されたMan5GlcNAc2にマンノース残基をalpha1,3-結合で転移する機能をもつ．この酵素は糖鎖を保持する真核生物に高度に保存されているにも関わらず，植物細胞では遺伝子，及び機能同定がなされていなかった．本報ではシロイヌナズナに存在するALG3のクローニング，機能同定を行い，植物細胞内における糖鎖修飾合成経路を明らかにした．またシロイヌナズナALG3欠損変異体では野生型には存在しない糖鎖を保持するが，野生型と同様の生育を示した。ヒトでは糖鎖修飾不全が重篤な疾病となるが、植物の場合必ずしも生育に影響を及ぼさないことを明らかにした．（文責；　梶浦裕之）

K. Dohi, J. Isoyama-Tanaka, T. Tokuda, and K. Fujiyama
Recombinant Expression and Characterization of N-Acetylglucosaminyltransferase I Derived from Nicotiana tabacum.
J Biosci Bioeng., 2010 109(4): 388-391.

N-アセチルグルコサミン転移酵素I（GnTI）は、N結合型糖鎖修飾経路において高マンノース型糖鎖から複合型糖鎖を生合成する際の鍵となる酵素である。しかしながら高等植物由来GnTIの生化学的諸性質には不明な点が多かった。本報ではタバコ由来GnTIをアフィニティタグとの融合タンパク質として大腸菌で発現し、活性を保持した状態で精製した。さらにこの精製酵素を用い、至適温度・基質特異性等の酵素学的諸性質を明らかにした。（文責；特任研究員　土肥浩二）

MFM. Khalil, H. Kajiura, K. Fujiyama, K. Koike, N. Ishida, and N. Tanaka
Tobacco plants overexpressing human UDP-galactose transporter gene hUGT1 display enhanced growth and the gibberellin-responsive phenotype
J Biosci Bioeng., 2010 109(2): 159-169.

H. Kajiura, H. Koiwa, Y. Nakazawa, A. Okazawa, A. Kobayashi, T. Seki, and K. Fujiyama.
Two Arabidopsis thaliana Golgi {alpha}-mannosidase Is are responsible for plant N-glycan maturation.
Glycobiology., 2010 20(2): 235-247.

{alpha}-mannosidase I（MANI）は小胞体からゴルジ体に輸送されてきた糖鎖の?1,2-結合したマンノース残基を加水分解する酵素である．MANIは動物細胞をはじめとする多くの生物種で保存された酵素であるにも関わらず，植物細胞においてはクローニング，及び機能同定がされていなかった．本報ではシロイヌナズナが２種類のMANI（AtMANI）を保持することを見出し，さらに細胞内局在，酵素学的性質を明らかにした．また両遺伝子欠損変異体シロイヌナズナの糖鎖構造を解析することで，シロイヌナズナ内におけるマンノース残基の加水分解は２種類のAtMANIにより担われていることを証明した。（文責；　梶浦裕之）

R. Kaewwichian, W. Yongmanitchai, N. Srisuk, K. Fujiyama, and S. Limyong.
Geotrichum siamensis sp. nov. and Geotrichum phurueaensis sp. nov., two asexual arthroconidial yeast species isolated in Thailand.
FEMS Yeast Res., 2010 10, 214-220.

WD. Kim, M. Tokunaga, H. Ozaki, T. Ishibashi, K. Honda, H. Kajiura, K. Fujiyama, R. Asano, I. Kumagai, T. Omasa, and H. Ohtake.
Glycosylation pattern of humanized IgG-like bispecific antibody produced by recombinant CHO cells.
Appl Microbiol Biotechnol., 2010 85(3): 535-542.

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2009年

S. Takashima, J. Seino, T. Nakano, K. Fujiyama, M. Tsujimoto, N. Ishida, and Y. Hashimoto
Analysis of CMP-sialic acid transporter-like proteins in plants.
Phytochemistry, 2009 70(17-18), 1973-1981.

R. Misaki, H. Kajiura, K. Fujii, K. Fujiyama, and T. Seki
Cloning and Characterization of Cytidine Monophosphate-3-Deoxy-D-Manno-Octulosonate Synthetase from Arabidopsis thaliana.
J Biosci Bioeng., 2009 108(6), 527-529.

グラム陰性菌リポ多糖に含まれる3-deoxy-D-manno-octulosonate (KDO)は菌の成長に必須の糖である。一方で、植物は細胞壁にKDOを持つもののその代謝経路や役割はほとんど分かっていない。本報では、植物におけるKDOの代謝・合成経路を明らかにする第一歩として、グラム陰性菌由来とは異なる酵素学的性質（至適pHなど）を持つcytidine monophosphate (CMP)-KDO合成酵素をシロイヌナズナより見出した。 （文責；助教　三崎亮）

K. Nakamura, M. Inoue, M. Maeda, R. Nakano, K. Hosoi, K. Fujiyama, and Y. Kimura.
Molecular cloning and gene expression analysis of tomato endo-beta-N-acetylglucosaminidase, an endoglycosidase involved in the production of high-mannose type free N-glycans during tomato fruit ripening.
Biosci Biotechnol Biochem., 2009 73(2), 461-464.

S. Limtong, W. Yongmanitchai, H. Kawasaki, and K. Fujiyama.
Wickerhamomyces edaphicus sp. nov. and Pichia jaroonii sp. nov., two ascomycetous yeast species isolated from forest soil in Thailand.
FEMS Yeast Res., 2009 9, 504-510.

N. Matoba, H.Kajiura, I. Cherni, J. D Doran, M. Bomsel, K. Fujiyama and T. S Mor.
Biochemical and Immunological Characterization of the plant-derived candidate HIV-1 mucosal Vaccine CTB MPR 649-684.
Plant Biotechnol J., 2009 7, 129-145.
See also the front cover page.

K. Fujiyama, Y. Sakai, R. Misaki,T. Omasa, and T. Seki.
Change in glycosylation pattern with extension of endoplasmic reticulum retention signal sequence of mouse antibody produced by suspension-cultured tobacco BY2 cells.
J Biosci Bioeng., 2009 107, 165-172.

M. Kilcoyne, M. Shah, J. Q Gerlach, V. Bhavanandan, V. Nagaraj, A. D Smith, K. Fujiyama, U. Sommer, C. E Costello, N. Olszewski, and L. Joshi.
O-glycosylation of protein subpopulations in alcohol-extracted rice proteins.
J Plant Physiol., 2009 166(3), 219-232.

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2008年

J.S. Kang, J. Frank, C.H. Kang, H. Kajiura, M. Vikram, A. Ueda, S. Kim, J.D. Bahk, B. Triplett, K. Fujiyama, S.Y. Lee, A. von Schaewen, and H. Koiwa.
Salt tolerance of Arabidopsis thaliana requires maturation of N-glycosylated proteins in the Golgi apparatus.
Proc, Natl, Acad, Sci, USA, 2008 105(15), 5933-5938.
   Erratum in Proc Natl Acad Sci USA., 2008 105(22), 7893

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2007年

Y. Kimura, T. Watanabe, M. Maeda, Y. Murata, and K. Fujiyama.
Salt-adaptation of Tobacco BY2 Cells Induces Change in Glycoform of N-Glycans: Enhancement of Exo- and Endo-glycosidase Activities by Salt-adaptation
Biosci Biotechnol Biochem., 2007 71 (4), 1111-1114.

L. Yang, H. Kajiura, K. Suzuki, S. Hirose, K. Fujiyama, and F. Takaiwa.
Generation of a transgenic rice seed-based edible vaccine against house dust mite allergy.
Biochem Biophys Res Commun., 2007 365, 334-339.

W. Saejung, K. Fujiyama, T. Takasaki, M. Ito, K. Hori, P. Malasit, Y. Watanabe, I. Kurane and T. Seki.
Production of dengue 2 envelope domain III in plant using TMV-based vector system.
Vaccine., 2007 25 (36), 6646-6654.

H. Tani, Y. Komoda, E. Matsuo, K. Suzuki, I. Hamamoto, T. Yamashita, K. Moriishi, K. Fujiyama, T. Kanto, N. Hayashi, A. Owsianka, A. H. Patel, M. A. Whitt, and Y. Matsuura.
Replication-Competent Recombinant Vesicular Stomatitis Virus Encoding Hepatitis C Virus Envelope Proteins.
J Virology., 2007 81(16), 8601-8612.

Y.Kimura, S. Sakamura, T. Ushijima, Y. Hama, H. Kajiura, K. Fujiyama, K. Okihara, K. Hashimoto, H. Sugimoto, and H. Yamada.
First evidence for b1-3 galactosyltransferase activity involved in biosynthesis of insect complex type N-glycan harboring T-antigen in Apis mellifera.
Biosci Biotechnol Biochem., 2007 71(4), 1111-1114.

K. Fujiyama, A. Furukawa, A. Katsura, R. Misaki, T. Omasa and Seki.
Production of mouse monoclonal antibody with galactose-extended sugar chain by suspension cultured tobacco BY2 cells expressing human b(1,4)-galactosyltransferase.
Biochem Biophys Res Commun., 2007 358(1), 85-91.

T. Okada, S, Ishiyama, H. Sezutsu, A. Usami,T. Tamura, K. Mita, K. Fujiyama, and T. Seki.
Molecular cloning and expression of two novel b-N-acetylglucosaminidases from Silkworm Bombyx mori .
Biosci Biotechnol Biochem., 2007 71(7), 1626-1635.

K. Fujiyama, R. Tanaka, M. Terashima, and T. Seki.
N-Linked glycan structures of glycoproteins in suspension-cultured Arabidopsis thaliana MM2d cells.
Plant Biotechnology., 2007 24, 255-259.

M.N. Cahyanto, H. Kawasaki, M. Nagashio, K. Fujiyama and T. Seki.
Construction of Lactobacillus plantarum strain with enhanced L-lysine yield.
J Appl Microbiol., 2007 102, 674-679.

藤山和仁
「植物細胞による組換えタンパク質生産：培養細胞は閉鎖型バイオリアクター」
化学と生物 2007年　Vo. 45, No. 7, 458-460.

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2006年

W. Saejung, C. Puttikhunt, T. Prommool, P. Sojikul, R. Tanaka, K. Fujiyama, P. Malasit, and T. Seki.
Enhancement of recombinant soluble dengue virus 2 envelope domain III protein production in Escherichia coli trxB and gor double mutant.
J Biosci Bioeng., 2006 102, 333-339.

K. Fujiyama, W. Saejung, I. Yanagihara, J. Nakado, R. Misaki, T. Honda, Y. Watanabe, and T. Seki.
In planta production of the immunogenic poliovirus peptide using TMV-based vector system.
J Biosci Bioeng., 2006 101, 398-402.

M. N. Cahyanto, H. Kawasaki, M. Nagashio, K. Fujiyama and T. Seki.
Regulation of aspartokinase, aspartate semialdehyde dehydrogenase, dihydrodipicolinate synthase, and dihydrodipicolinate reductase in Lactobacillus plantarum.
Microbiology., 2006 152, 105-112.

R. Misaki, K. Fujiyama and T. Seki.
Expression of human CMP-N-acetylneuraminic acid synthetase, and CMP-sialic acid transporter in tobacco suspension-cultured cell.
Biochem Biophys Res Commun., 2006 339, 1184-1189.

K. Fujiyama, R. Misaki, A. Katsura, T. Tanaka, A. Furukawa, T. Omasa and T. Seki.
N-Linked glycan structures of a mouse monoclonal antibody produced from tobacco BY2 suspension-cultured cells.
J Biosci Bioeng., 2006 101, 212-218.

藤山和仁
「組換え医療用糖タンパク質の生産に向けた宿主細胞の育種」
酵素工学ニュース 2006年 第55号 17-23.

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2005年

M. Nur Cahyanto, H. Kawasaki, K. Fujiyama and T. Seki.
Cloning of IAM 12477 lysine biosynthetic genes encoding functional aspartate semialdehyde dehydrogenase, dihydrodipicolinate synthase, and dihydrodipicolinate reductase.
World J of Microbiol Biotechnol., 2006 22, 409-416.

S. Nandi, D. Yalda, S. Lu, Z. Nikolov, R. Misaki, K. Fujiyama and N. Huang.
Process development and economic evaluation of recombinant human lactoferrin expressed in rice grain.
Transgenic Research, 2005 14, 237-249.

藤山和仁
「植物バイオリアクターによる外来タンパク質生産　-特集によせて-」
生物工学会誌 第83号 第11号 509 (2006)
「植物生産医療タンパク質と糖鎖」
生物工学会誌 2006年　第83号 第11号 522-524.

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2004年

K. Fujiyama, Y. Sakai, R. Misaki, I. Yanagihara, T. Honda, H. Anzai and T. Seki.
N-linked glycan structures of human lactoferrin produced by rice.
Biosci Biotechnol Biochem., 2004 68, 2565-2570.

藤山和仁
「分子農業を目指した植物糖鎖工学」
バイオサイエンスとバイオインダストリー 2004年 Vo. 62, No. 12, 19-24.

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2003年

R, Misaki. Y, Kimura. N. Q. Palacpac, S, Yoshida. K, Fujiyama. T. Seki.
Plant cultured cells expressing human beta1,4-galactosyltransferase secrete glycoproteins with galactose-extended N-linked glycans.
Glycobiology., 2003 13, 199-205.

R. Misaki, K. Fujiyama, H. Yokoyama, Y. Ido, K. Miyauchi, T. Yoshida, and T. Seki.
Characterization of almond a-mannosidase and its application for structureanalysis of sugar chain.
J Biosci Bioeng., 2003 96, 187-192.

R. Misaki, H. Nagaya, K. Fujiyama, I, Yanagihara, T. Honda, and T. Seki.
N-linked glycan structure of mouse interferon-b produced by Bombyx mori larvae.
Biochem Biophys Res Commun., 2003 311, 979-986.

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2001年

K. Fujiyama, Y. Ido, R. Misaki, D. G. Moran, I. Yanagihara, T. Honda, S. Nishimura, T. Yoshida and T. Seki.
Human N-acetylglucosaminyltransferase I. Expression in E. coli as a soluble enzyme, and application as an immobilized. enzyme for the chemoenzymatic synthesis of N-linked oligosaccharides.
J Biosci Bioeng., 2001 92, 569-574.

K. Fujiyama, Y. Kira, M. Iizuka, Y. Kimura and T. Seki.
Identification of putative gene encoded on ORF16 of the 81 kb contig of Arabidopsis thaliana chromosome III as a-mannosidase.
J Biosci Bioeng., 2001 92, 401-404.

R. Misaki, Y. Kimura, K. Fujiyama and T. Seki.
Glycoproteins Secreted from Suspension-cultured Tobacco BY2 Cells have Distinct Glycan Structures from Intracellular Glycoproteins.
Biosci Biotechnol Biochem., 2001 65, 2482-2488.

K. Fujiyama, S. Sakuradani, D. G. Moran, T. Yoshida and T. Seki.
Effect of a-1,2-mannosidic linkage located in a1,3-branch of Man6GlcNAc2 oligosaccharide on enzyme activity of recombinant human Man9-mannosidase produced in Escherichia coli.
J Biosci Bioeng., 2001 91, 419-421.

S. Shibatani, K. Fujiyama, S. Nishiguchi, T. Seki and Y. Maekawa.
Production and characterization of an active and soluble human b1,4-galactosyltransferase in Escherichia coli as a useful catalyst in the synthesis of Galb1-->4 GlcNAc linkage.
J Biosci Bioeng., 2001 91, 85-87.

K. Fujiyama,N. Q. Palacpac, H, Sakai. Y, Kimura. A, Shinmyo. T, Yoshida. T. Seki
In vivo conversion of a glycan to human compatible type by transformed tobacco cells.
Biochem Biophys Res Commun., 2001 289, 553-557.

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2000年

W. Panbangred, K. Weeradechapon, S. Udomvaraphant, K. Fujiyama and V. Meevootisom.
High expression of penicillin G acylase gene (pac) from Bacillus megaterium UN1 in its pac minus mutant.
J. Appl. Microbiol., 2000 89 , 152-157.

1999年

T. Okuda, A. G. Pue, K. Fujiyama, and K. Yokotsuka.
Purification and characterization of polyphenol oxidase from muscat bailey A grape juice.
American J. Enol. Viticul., 1999 50, 137-143.

N. Q. Palacpac, S. Yoshida, H. Sakai, Y. Kimura, K. Fujiyama, T. Yoshida and T. Seki.
Stable expression of human b1,4-galactsosyltransferase gene in plant cells modifies N-glycosylation.
Proc. Natl. Acad. Sci. USA, 1999 96, 4692-4697.

N. Q. Palacpac, Y. Kimura, K. Fujiyama, T. Yoshida and T. Seki.
Structures of N-linked oligosaccharides of glycoproteins from tobacco BY2 suspension cultured cells.
Biosci. Biotechnol. Biochem., 1999 63, 35-39.

1998年

S. Teeradakorn, Y. Talawanich, I. Suzuki, P. Pinphanichakarn, K. Fujiyama,T. Seki, and T. Yoshida.
Characterization of Streptomyces D3 derived from protoplast fusion between Streptomyces cyaneus 190-1 and Streptomyces. griseoruber 42-9.
World J. Microbiol. Biotechnol., 1998 14, 565-570.

D. G. Moran, S. Sakuradani, K. Fujiyama, T. Seki and T. Yoshida.
Characterization of recombinant human Man9-mannosidase expressed in Escherichia coli.
J. Ferment. Bioeng., 1998 86, 277-283.

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