大阪大学生物工学国際交流センター 藤山研究室  
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業績

【2019年10月31日更新】

::最近5年間の発表論文 ::

PubMedに記載されている論文についてはPubMedIDにリンクしています。

2019年

Fujiyama, K. (2019)..
‘Plant and Glycan’, in Taniguchi, N., et al. (eds.) Glycoscience: Basic Science to Applications. Singapore: Springer, pp. 114-115.

Matsuura H, Ujiie K, Duyen TTM, Izutsu K, Fujiyama K, Maeda S, Harada K, Hirata K.
Development of a Paper-Based Luminescence Bioassay for Therapeutic Monitoring of Aminoglycosides: a Proof-of-Concept Study.
Appl Biochem Biotechnol. 2019 May 23 doi: 10.1007/s12010-019-03048-4.

Sakaoduoen Bunsangiam, Varunya Sakpuntoon, Nantana Srisuk, Takao Ohashi , Kazuhito Fujiyama & Savitree Limtong
Biosynthetic Pathway of Indole-3-Acetic Acid in Basidiomycetous Yeast Rhodosporidiobolus fluvialis
Mycobiology 23 June 2019 doi:10.1080/12298093.2019.1638672

Arofatullah NA, Kabirun S, Fujiyama K, Widianto D
Molecular identification and in vitro propagation of arbuscular mycorrhiza from tea plant rhizosphere
Current Research in Environmental & Applied Mycology 9(1): 92–102 (2019)

*本論文に示されている成果の一部は、Arofatullah NAがBiotechnology School in Asia programに在籍中になされてたものである

2018年

Fujiyama, K. (2018).
‘Glycoprotein Biosynthesis of Silkworm’, in Maenaka, K. and Park, E.Y. (eds.) Silkworm Biofactory. Boca Raton: CRC Press, pp. 107-126.

Ohashi H, Ohashi T, Misaki R, Fujiyama K.
Arabidopsis thaliana α1,2-l-fucosyltransferase catalyzes the transfer of l-galactose to xyloglucan oligosaccharides.
FEBS Lett. 2019 Jan;593(2):187-194

Nakamura M, Tran ML, Le QL, Suzuki H, Nguyen CM, Vu HG, Tamura K, Nguyen VT, Suzuki H, Misaki R, Muranaka T, Ninh KB, Fujiyama K, Seki H.
Transcriptome sequencing and identification of cytochrome P450 monooxygenases involved in the biosynthesis of maslinic acid and corosolic acid in Avicennia marina
Plant Biotechnology, 35, 1–8 (2018)

Tsai YY, Ohashi T, Wu CC, Bataa D, Misaki R, Limtong S, Fujiyama K.
Delta-9 fatty acid desaturase overexpression enhanced lipid production and oleic acid content in Rhodosporidium toruloides for preferable yeast lipid production.
J Biosci Bioeng., 2018 Oct 10. pii: S1389-1723(18)30688-1. doi: 10.1016/j.jbiosc.2018.09.005. [Epub ahead of print]

藤山和仁
カイコの高次な生物機能の“革命的”利用、研究ノート
生産と技術、第70巻、第4号、65-8 (2018)

Flores RJD, Ohashi T, Sakai K, Gonoi T, Kawasaki H, Fujiyama K.
The neutral N-linked glycans of the Basidiomycetous yeasts Pseudozyma antarctica and Malassezia furfur (Subphylum Ustilaginomycotina).
J Gen Appl Microbiol., 2018 Oct 10. doi: 10.2323/jgam.2018.05.003. [Epub ahead of print]

Wu CC, Tsai YY, Ohashi T, Misaki R, Limtong S, Fujiyama K.
Isolation of a thermotolerant Rhodosporidium toruloides DMKU3-TK16 mutant and its fatty acid profile at high temperature.
FEMS Microbiol Lett., 2018 Aug 17. doi: 10.1093/femsle/fny203. [Epub ahead of print]

Priyambada SA, Misaki R, Okamoto T, Ohashi T, Ueda K, Matsuura Y, Fujiyama K.
St6gal1 knockdown alters HBV life cycle in HepAD38 cells.
Biochem Biophys Res Commun., 2018 Sep 10; 503(3): 1841-1847

Naphatsamon U, Ohashi T, Misaki R, Fujiyama K.
The Production of Human β-Glucocerebrosidase in Nicotiana benthamiana Root Culture.
Int J Mol Sci., 2018 Jul 6; 19(7)

大橋貴生
機能性フラボノイド配糖体の醗酵生産、研究ノート
生産と技術、第70巻、第1号、103-5 (2018)

Suganuma M, Nomura T, Higa Y, Kataoka Y, Hunaguma S, Okazaki H, Suzuki T, Fujiyama K, Sezutsu H, Tatematsu K, Tamura T.
N-glycan sialylation in a silkworm-baculovirus expression system
J Biosci Bioeng., 2018 Jul; 126(1): 9-14.

Nguyen TS, Misaki R, Ohashi T, Fujiyama K.
Enhancement of glycosylation by stable co-expression of two sialylation related enzymes on Chinese hamster ovary cells
J Biosci Bioeng., 2018 Jul; 126(1): 102-110

Roychowdhury S, Youngjun Oh, Kajiura H, Hamorsky K, Fujiyama K, Matoba N.
Hydroponic treatment of Nicotiana benthamiana with kifunensine modifies the N-glycans of recombinant glycoprotein antigens to predominantly Man9 high-mannose type upon transient overexpression
Front. Plant Sci., 2018 Jan 30; 9: 62

::学会発表 2018年度:

2017年

大橋貴生
高等植物のペクチン生合成および分解に関与する糖質関連酵素群、今日の話題
化学と生物、第55巻、第7号、454-6 (2017)

大橋貴生
酵母ベクターの種類と歴史、続・生物工学基礎講座 バイオよもやま話
生物工学会誌、第95巻、第1号、16-9 (2017)

Polburee P, Ohashi T, Tsai YY, Sumyai T, Lertwattanasakul N, Limtong S, Fujiyama K.
Molecular cloning and overexpression of DGA1, an acyl-CoAdependent diacylglycerol acyltransferase, in the oleaginous yeast Rhodosporidiobolus fluvialis DMKU-RK253
Microbiology, 2018 Jan; 164(1): 1-10

Pierce OM, McNair GR, He X, Kajiura H, Fujiyama K, Kermode AR.
N-glycan structures and downstream mannose-phosphorylation of plant recombinant human alpha-L-iduronidase: toward development of enzyme replacement therapy for mucopolysaccharidosis I.
Plant Mol Biol., 2017 Dec; 95(6): 593-606

Ohashi H, Wahl C, Ohashi T, Elling L and Fujiyama K.
Effective synthesis of guanosine 5'-diphospho-β-L-galactose using bacterial L-fucokinase/guanosine 5'-diphosphate-L-fucose pyrophosphorylase
Advanced Synthesis & Catalysis, DOI: 10.1002/adsc.201700901

Ishinishi R, Matsuura H, Tanaka S, Nozawa S, Tanada K, Kawashita N, Fujiyama K, Miyasaka H, Hirata K.
Isolation and characterization of a stress-responsive gene encoding a CHRD domain-containing protein from a halotolerant green alga.
Gene, 2018 Jan 15; 640:14-20

Priyambada SA, Misaki R, Okamoto T, Okamoto Y, Ohashi T, Ueda K, Matsuura Y, Fujiyama K.
Cell surface N-glycan alteration in HepAD38 cell lines expressing Hepatitis B virus.
Virus Res., 2017 Jun 20; 238: 101-109.

Kajiura H, Suzuki N, Tokumoto Y, Yoshizawa T, Takeno S, Fujiyama K, Kaneko Y, Matsumura H, Nakazawa Y.
Two Eucommia farnesyl diphosphate synthases exhibit distinct enzymatic properties leading to end product preferences.
Biochimie, 2017 Aug; 139: 95-106.

Flores RJ, Ohashi T, Kawasaki H, Fujiyama K.
The Neutral N-linked Glycans of the Ustilaginomycete Yeast Sympodiomycopsis paphiopedili.
Yeast, 2017 Jul; 34(7): 305-317.

::学会発表 2017年度:

2016年

Ohashi T, Jinno J, Inoue Y, Ito S, Fujiyama K, Ishimizu T.
A polygalacturonase localized in the Golgi apparatus in Pisum sativum.
J Biochem, 2017 Sep 1;162(3):193-201.

*立命館大 石水先生等との共同研究

Uehara Y, Tamura S, Maki Y, Yagyu K, Mizoguchi T, Tamiaki H, Imai T, Ishii T, Ohashi T, Fujiyama K, Ishimizu T.
Biochemical characterization of rhamnosyltransferase involved in biosynthesis of pectic rhamnogalacturonan I in plant cell wall.
Biochem Biophys Res Commun, 2017 Apr 22;486(1):130-136.

*立命館大 石水先生等との共同研究

Ohashi H, Ohashi T, Kajiura H, Misaki R, Kitamura S, Fujiyama K.
Fucosyltransferases produce N-glycans containing core L-galactose.
Biochem Biophys Res Commun, 2017 Jan 29;483(1):658-663

*大阪府立大 北村先生との共同研究

De Martinis D, Rybicki EP, Fujiyama K, Franconi R, Benvenuto E.
Editorial: Plant Molecular Farming: Fast, Scalable, Cheap, Sustainable.
Front Plant Sci, 2016 Aug 3;7:1148.

Misaki R, Fukura N, Kajiura H, Yasugi M, Kubota-Koketsu R, Sasaki T, Momota M,Ono K, Ohashi T, Ikuta K, Fujiyama K.
Recombinant production and characterization of human anti-influenza virus monoclonal antibodies identified from hybridomas fused with human lymphocytes.
Biologicals, 44(5); 394-402 (2016)
*SATRERSでの研究成果

Tsai YY, Ohashi T, Kanazawa T, Polburee P, Misaki R, Limtong S, Fujiyama K.
Development of a sufficient and effective procedure for transformation of an oleaginous yeast, Rhodosporidium toruloides DMKU3-TK16
Curr Genet., 2017 May; 63(2): 359-371.

*タイ・カセサート大との共同研究

Takamatsu S, Shimomura M, Kamada Y, Maeda H, Sobajima T, Hikita H, Iijima M, Okamoto Y, Misaki R, Fujiyama K, Nagamori S, Kanai Y, Takehara T, Ueda K, Kuroda S, Miyoshi E.
Core-fucosylation plays a pivotal role in hepatitis B pseudo virus infection: a possible implication for HBV glyco-therapy.
Glycobiology., 2016 Nov;26(11):1180-1189

*医学部、産研との共同研究(厚労科研)

Pirapan Polbureea P, Yongmanitchaia W, Honda K, Ohashi T, Yoshida T, Fujiyama K, Limtonga S
Lipid production from biodiesel-derived crude glycerol by Rhodosporidium fluviale DMKU-RK253 using temperature shift with high cell density
Biochem Eng J., 112, 208-218(2016)
*タイ・カセサート大との共同研究

Limkul J, Iizuka S, Sato Y, Misaki R, Ohashi T, Ohashi T, Fujiyama K.
The production of human glucocerebrosidase in glyco-engineered Nicotiana benthamiana plants.
Plant Biotechnol J., 14(8); 1682-1694 (2016)

*慈恵医大・大橋十也教授グループとの共同研究

Trempel F, Kajiura H, Ranf S, Grimmer J, Westphal L, Zipfel C, Scheel D, Fujiyama K and Lee J
Altered glycosylation of exported proteins, including surface immune receptors, compromises calcium and downstream signaling responses to microbe-associated molecular patterns in Arabidopsis thaliana.
BMC Plant Biol., 16(1): 31. doi: 10.1186/s12870-016-0718-3. (2016)

*ドイツLeibniz Institute of Plant Biochemistry(Halle/Saale)等との共同研究

::学会発表 2016年度:

2015年

Roongsawang N, Puseenam A, Kitikhun S, Sae-Tang K, Harnpicharnchai P, Ohashi T, Fujiyama K, Tirasophon W, Tanapongpipat S.
A Novel Potential Signal Peptide Sequence and Overexpression of ER-Resident Chaperones Enhance Heterologous Protein Secretion in Thermotolerant Methylotrophic Yeast Ogataea thermomethanolica.
Appl Microbiol Biotechnol., 178(4); 710-724 (2016)

   *タイ・国立研究所BIOTECとの共同研究

Ohashi T, Hasegawa Y, Misaki R, Fujiyama K.
Substrate preference of citrus naringenin rhamnosyltransferases and their application to flavonoid glycoside production in fission yeast.
Appl Microbiol Biotechnol.,100(2):687-696 (2015)

Polburee P, Yongmanitchai W, Lertwattanasakul N, Ohashi T, Fujiyama K, Limtong S.
Characterization of oleaginous yeasts accumulating high levels of lipid when cultivated in glycerol and their potential for lipid production from biodiesel-derived crude glycerol
Fungal Biol.,119(12):1194-1204 (2015)

  *タイ・カセサート大との共同研究

Limkul J, Misaki R, Kato K, Fujiyama K.
The combination of plant translational enhancers and terminator increase the expression of human glucocerebrosidase in Nicotiana benthamiana plants
Plant Science, 240: 41-49 (2015)

Kajiura H, Hamaguchi Y, Mizushima H, Misaki R, Fujiyama K.
Sialylation potentials of the silkworm, Bombyx mori; B. mori possesses an active α2,6-sialyltransferase.
Glycobiology, 25(12): 1441-1453 (2015)

Arief II, Jenie BSL, Astawan M, Fujiyama K, Witarto AB.
Identification and probiotic characteristics of lactic acid bacteria isolated from Indonesian local beef
Asian J Animal Sciences, 9 (1): 25-36 (2015)

  *インドネシア・ボコール農大との共同研究

藤山和仁
「第4編 第3章 糖鎖の合成-生物系 第3節 植物を利用した糖タンパク質合成」
糖鎖の新機能開発・応用ハンドブック 監修:秋吉一成

株式会社 NTS, 2015年8月

H. Kajiura, K. Fujiyama
Im“plant”ing of Mammalian Glycosyltransferase Gene into Plant Suspension-Cultured Cells Using Agrobacterium-Mediated Transformation
Methods Mol Biol.,1321: 225-232 (2015)

Hamorsky KT, Kouokam JC, Jurkiewicz JM, Nelson B, Moore LJ, Husk AS, Kajiura H, Fujiyama K, Matoba N.
N-glycosylation of cholera toxin B subunit in Nicotiana benthamiana: impacts on host stress response, production yield and vaccine potential.
Sci Rep., 5:8003. doi: 10.1038/srep08003 (2015)

Srichaisupakit A, Ohashi T, Misaki R, Fujiyama K.
Production of the Initial-Stage Eukaryotic N-Glycan and Protein Glycosylation in Escherichia coli.
J Biosci Bioeng., 119(4): 399-405 (2015)

Nomura T, Suganuma M, Higa Y, Kataoka Y, Funaguma S, Okazaki H, Suzuki T, Kobayashi I, Sezutsu H, Fujiyama K.
Improvement of glycosylation structure by suppression of β-N-acetylglucosaminidases in silkworm.
J Biosci Bioeng., 119(2): 131-136 (2015)

::学会発表 2015年度:

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2014年

Fujiyama K.
Heterologous Glycoprotein Production (Plant)
In: Glycoscience: Biology and Medicine. Naoyuki Taniguchi,Tamao Endo, Gerald W. Hart, Peter H. Seeberger, Chi-Huey Wong (Ed.) Springer Pages 1545-1549 ISBN: 978-4-431-54840-9 (Print) 978-4-431-54841-6 (Online)., 2014 March.

梶浦裕之, 藤山和仁
植物N-結合型糖鎖修飾酵素の異種細胞における発現と修飾経路の考察」
化学と生物 Vol.52 No.11 719-724 (2014)

Polburee P, Yongmanitchai W, Ohashi T, Fujiyama K, Limtong S.
Barnettozyma siamensis f.a., sp. nov., a novel lipid accumulating ascomycete yeast species isolated in Thailand.
Int J Syst Evol Microbiol., 64(Pt 9): 3053-3057 (2014)

Yumioka-Ito H, Misaki R, Yokoro M, Suzuki M, Yamashita H, Hiemori-Kondo M, Kimoto M, Kato K, Fujiyama K, Tsuji H.
Cloning of a cDNA encoding the Gly m Bd 28K precursor and its vacuole transport in tobacco BY2 suspension-cultured cells.
J Nutr Sci Vitaminol., 60(2): 129-139 (2014)

Pitaksajjakul P, Benjathummarak S, Pipattanaboon C, Wongwit W, Okabayashi T, Kuhara M, Misaki R, Fujiyama K, Ramasoota P.
Antibody Germline Characterization of Cross-neutralizing human IgGs against 4 serotypes of Dengue virus.
Biochem Biophys Res Commun., 446(2): 475-80 (2014)

Srichaisupakit A, Ohashi T, Fujiyama K.
Identification of a protein glycosylation operon from Campylobacter jejuni JCM 2013 and its heterologous expression in Escherichia coli.
J Biosci Bioeng., 118(3): 256-262 (2014)

::学会発表 2014年度:

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2013年

Yasugi M, Kubota-Koketsu R, Yamashita A, Kawashita N, Du A, Misaki R, Kuhara M, Boonsathorn N, Fujiyama K, Okuno Y, Nakaya T, Ikuta K.
Emerging Antigenic Variants at the Antigenic Site Sb in Pandemic A(H1N1)2009 Influenza Virus in Japan Detected by a Human Monoclonal Antibody.
PLoS One., 8(10): e77892 (2013)

Kajiura H, Wasai M, Kasahara S, Takaiwa F, Fujiyama K.
N-Glycosylation and N-glycan moieties of CTB expressed in rice seeds.
Mol Biotechnol., Jul;54(3): 784-794
(2013)

藤山和仁
「糖鎖プロセシング酵素、マンノシダーゼ」
応用糖質科学 第3巻 第1号 87-92 (2013)

Wang S, Takahashi H, Kajiura H, Kawakatsu T, Fujiyama K, Takaiwa F.
Transgenic Rice Seeds Accumulating Recombinant Hypoallergenic Birch Pollen Allergen Bet v 1 Generate Giant Protein Bodies.
Plant Cell Physiol., 54(6): 917-933 (2013)

Sasaki T, Setthapramote C, Kurosu T, Nishimura M, Asai A, Omokoko MD, Pipattanaboon C, Pitaksajjakul P, Limkittikul K, Subchareon A, Chaichana P, Okabayashi T, Hirai I, Leuangwutiwong P, Misaki R, Fujiyama K, Ono K, Okuno Y, Ramasoota P, Ikuta K.
Dengue virus neutralization and antibody-dependent enhancement activities of human monoclonal antibodies derived from dengue patients at acute phase of secondary infection.
Antiviral Res., 98(3): 423-431 (2013)

Hamorsky KT, Kouokam JC, Bennett LJ, Baldauf KJ, Kajiura H, Fujiyama K, Matoba N.
Rapid and scalable plant-based production of a cholera toxin B subunit variant to aid in mass vaccination against cholera outbreaks.
PLoS Negl Trop Dis., 7(3): e2046 (2013)
 

University of Louisville School of Medicineとの共同研究です。Nicotiana benthamianaを使い、 cholera toxin B subunit の効率的生産に関して調査しました。

Yasugi M, Kubota-Koketsu R, Kawashita N, Anariwa D, Misaki R, Kuhara M, Boonsathorn N, Fujiyama K, Okuno S, Nakaya T, Ikuta K.
Human Monoclonal Antibodies Broadly Neutralizing against Influenza B Virus.
PLoS Pathog., 9(2): e1003150 (2013)

大阪大学微生物病研究所等との共同研究の成果です。
インフルエンザBウィルスに対するヒト由来の抗体について、その遺伝子構造・性状に関して明らかにしました。

Watanabe S, Kakudo A, Ohta M, Mita K, Fujiyama K, Inumaru S.
Molecular cloning and characterization of the alpha-glucosidase II from Bombyx mori and Spodoptera frugiperda.
Insect Biochem Mol Biol., 43(4): 319-327 (2013)

(独)動物衛生研究所との共同研究の成果です。
カイコのエサであるクワにはグルコシダーゼ阻害物質が含まれているにも関わらず、カイコは問題なく摂取しています。カイコよりグルコシダーゼ遺伝子を単離し、阻害剤抵抗性を調査しました。

::学会発表 2013年度:

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::2012年以前の発表論文::

Copyright (C) Fujiyama lab., ICBiotech, Osaka University