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【2022年11月4日更新】

::最近5年間の発表論文::

PubMedに記載されている論文についてはPubMedIDにリンクしています。

2022年

Kajiura H, Tatematsu K, Nomura T, Miyazawa M, Usami A, Tamura T, Sezutsu H and Fujiyama K
Insights into the quality of recombinant proteins produced by two different Bombyx mori expression systems
Sci. Rep., 2022 Nov 12(1):18502.

Macauyag EA, Kajiura H, Ohashi T, Misaki R and Fujiyama K
High-level transient production of a protease-resistant mutant form of human basic fibroblast growth factor in Nicotiana benthamiana leaves
Plant Biotech., 2022 39(3):291-301

Yamamoto Y, Kajiura H, Nishibu T and Fujiyama K
Mamestra brassicae NIAS-Mb-32 cell strain 2g2 enables high-yield recombinant protein production in baculovirus-free and baculovirus-based insect cell expression
J Biosci. Bioeng., 2022 In Press, Corrected Proof, Available online 24 September 2022

Dent M, Mayer KL, Garcia NV, Guo H, Kajiura H, Fujiyama K and Matoba N
Impact of glycoengineering and anti-drug antibodies on the anti-cancer activity of a plant-made lectin-Fc fusion protein
Plant Biotechnol J., 2022 Jul 28

Kurniati A, Puspaningsih NNT, Putri KDA, Damayanti M, Purwani NN, Rahmah SA, Purkan, Fujiyama K, Sakka M, Sakka K, Kimura T, Rohman A, Baktir A and Sanjayaadg RE
Heterologous fusion gene expression and characterization of a novel carbohydrate binding module (Cbm36) to laccase (Lcc2)
Biocatal. Atricul. Biotech., 2022 Jul 42:102377.

Kajiura H, Eguchi T, Uchino K, Tatematsu K, Tamura T and Sezutsu H
Temporal analysis of N-acetylglucosamine extension of N-glycans in the middle silk gland of silkworm Bombyx mori
J Biosci. Bioeng., 2022 Jun 133(6):533-540.

Kajiura H, Hiwasa-Tanase K, Ezura H, Fujiyama K
Effect of fruit maturation on N-glycosylation of plant-derived native and recombinant miraculin
Plant Physiol Biochem., 2022 May 178:70-79

Uthailak N, Kajiura H, Misaki R, Fujiyama K
Production of recombinant β-glucocerebrosidase in wild-type and glycoengineered transgenic Nicotiana benthamiana root cultures with different N-glycan profiles.
Plant Physiol Biochem., 2022 May 133(5):481-488

::学会発表 2022年度:

 

2021年

Misaki R, Iwasaki M, Takechi H, Yamano-Adachi N, Ohashi T, Kajiura H, Fujiyama K
Establishment of serum-free adapted Chinese hamster ovary cells with double knockout of GDP-mannose-4,6-dehydratase and GDP-fucose transporter.
Cytotechnology, 2022 Feb 74(1):163-179.

Uthailak N, Kajiura H, Misaki R, Fujiyama K
Production of recombinant b-glucocerebrosidase in wild-type and glycoengineered transgenic Nicotiana benthamiana root cultures with different N-glycan profiles
J Biosci. Bioeng., 2022 Feb 18;S1389-1723(22)00002-0.

Ohashi T, Sari N, Misaki R, Fujiyama K.
Biochemical characterization of Arabidopsis clade F polygalacturonase shows a substrate preference toward oligogalacturonic acids.
J Biosci. Bioeng. 2022 Jan 133(1):1-7

藤山和仁
植物を用いた医療用タンパク質生産
Applied Cell Biology Japan 2021 34:17-32

Sariyatun R, Kajiura H, Limkul J, Misaki R, Fujiyama K.
Analysis of N-glycan profile of Arabidopsis alg3 cell culture.
Plant Biotechnol (Tokyo)., 2021 Dec 25;38(4):463-467

Chan JLR. , Leon MP. , Quimio JMUP., Fujiyama K, Sabino NG., and Mendoza BC.
The Potential Bacteriocinogenic Activity of Putative Bacteriocins of E. coli from Bat Guano against Enterohemorrhagic E. coli Strains
Philippine Journal of Veterinary Medicine Vol. 58, No. 3

Yamamoto C, Ogura M, Uemura R, Megumi M, Kajiura H, Misaki R, Fujiyama K, Kimura Y.
Improved assay system for acidic peptide: N-glycanase (aPNGase) activity in plant extracts.
Anal. Biochem. 2021 Sep 9:114367. doi: 10.1016/j.ab.2021.114367.

Sariyatun R, Florence, Kajiura H, Ohashi T, Misaki R, Fujiyama K.
Production of Human Acid-Alpha Glucosidase With a Paucimannose Structure by Glycoengineered Arabidopsis Cell Culture.
Front Plant Sci., 2021 Jul 14;12:703020.

藤山和仁
植物を用いたバイオリアクターによるワクチン、抗体の生産
PHARM STAGE 21(4): 18-22 2021年7月号

Uthailak N, Kajiura K, Misaki R, Fujiyama K
Transient Production of Human β-Glucocerebrosidase with Mannosidic-Type N-Glycan Structure in Glycoengineered Nicotiana benthamiana Plants
Front Plant Sci., 2021 Jun 7;12:683762.

Wu CC, Ohashi T, Kajiura H, Sato Y, Misaki R, Honda K, Limtong S, Fujiyama K
Functional characterization and overexpression of Δ12-desaturase in the oleaginous yeast Rhodotorula toruloides for production of linoleic acid-rich lipids.
J Biosci Bioeng., 2021 Jun 131(6):631-639.

Shirai S, Uemura R, Maeda M, Kajiura H, Misaki R, Fujiyama K, Kimura Y.
Direct evidence of cytosolic PNGase activity in Arabidopsis thaliana: in vitro assay system for plant cPNGase activity.
Biosci Biotechnol Biochem., 2021 May 25;85(6):1460-1463.

Kajiura H, Miyauchi R, Kakudo A, Ohashi T, Misaki R, Fujiyama K
Bombyx mori β1,4-N-acetylgalactosaminyltransferase possesses relaxed donor substrate specificity in N-glycan synthesis
Scientific Reports, 2021 Mar 9;11(1):5505.

Kajiura H, Yoshizawa T, Tokumoto Y, Suzuki N, Takeno S, Jumtee-Takeno K, Yamashita T, Tanaka S, Kaneko Y, Fujiyama K, Matsumura H, Nakazawa Y
Structure-function studies of ultrahigh molecular weight isoprenes provide key insights into their biosynthesis
Commun Biol., 2021 Feb 16;4(1):215.

::学会発表 2021年度:

2020年

Gungupalli MP, Kajiura H, Ishimizu T, Bhattacharyya D
Alkaline hydrolysis of coconut pith: process optimization, enzymatic saccharification, and nitrobenzene oxidation of Kraft lignin
Biomass Conv. Bioref., 2020 https://doi.org/10.1007/s13399-020-00890-z

Wachananawat B, Kuroha T, Takenaka Y, Kajiura H, Naramoto S, Yokoyama R, Ishizaki K, Nishitani K, Ishimizu T.
Diversity of Pectin Rhamnogalacturonan I Rhamnosyltransferases in Glycosyltransferase Family 106
Front Plant Sci., 2020 Jul 2;11:997.

Arifin M, Budiman C, Fujiyama K, Arief II
Kinetic and Thermodynamic Study of Plantaricin IIA-1A5, a Bacteriocin Produced by Indonesian Probiotic Lactobacillus plantarum IIA-1A5
Protein Pept Lett., 2020 Nov doi: 10.2174/0929866527999201123213841.

Ohashi T, Fujisawa Y, Hayes MR, Misaki R, Pietruszka J, Fujiyama K
Transglycosylation toward naringenin-7-O-glucoside using an N180H mutant of Coprinopsis cinerea endo-β-N-acetylglucosaminidase
Biochem Biophys Res Commun., 2020 Sep 10; 530(1): 155-159.

Kajiura H, Nakamura Y, Nishimura M, Ohashi T, Misaki R, Fujiyama K
Characterization of Bombyx mori N-acetylglucosaminyltransferase II splicing variants
Biochem Biophys Res Commun., 2020 Aug 20;529 (2): 404-410.

Wongwisansri S, Promdonkoy P, Likhitrattanapisal S, Harnpichanchai P, Fujiyama K, Kaneko Y, Eurwilaichitr L, Ingsrisawang S, Tanapongpipat S
Mating-type switching and mating-type gene array expression in the methylotrophic yeast Ogataea thermomethanolica TBRC656
Microbiological Resarch, 2020 Feb 232:126372

Matsumura T, Amatsu S, Misaki R, Yutani M, Anariwa D, Kohda T, Fujiyama K, Ikuta K, Fujinaga Y
Fully Human Monoclonal Antibodies Effectively Neutralizing Botulinum Neurotoxin Serotype B
Toxins, 2020 May 7;12(5):302.

Nguyen TS, Misaki R, Ohashi T, Fujiyama K.
Enhancement of sialylation in rIgG in glyco-engineered Chinese hamster ovary cells.
Cytotechnology, 2020 Jun 72(3):343-355.

::学会発表 2020年度:

2019年

Fujiyama, K. (2019)
‘Plant and Glycan’, in Taniguchi, N., et al. (eds.) Glycoscience: Basic Science to Applications. Singapore: Springer,pp. 114-115.
Singare: Springer,

Matsuura H, Ujiie K, Duyen TTM, Izutsu K, Fujiyama K, Maeda S, Harada K, Hirata K.
Development of a Paper-Based Luminescence Bioassay for Therapeutic Monitoring of Aminoglycosides: a Proof-of-Concept Study.
Appl Biochem Biotechnol., 2019 Nov 189(3):798-809.

Bunsangiam S, Sakpuntoon V, Srisuk N, Ohashi T, Fujiyama K & Limtong S
Biosynthetic Pathway of Indole-3-Acetic Acid in Basidiomycetous Yeast Rhodosporidiobolus fluvialis
Mycobiology, 2019 Jul 15;47(3):292-300.

Flores RJD, Ohashi T, Sakai K, Gonoi T, Kawasaki H, Fujiyama K.
The neutral N-linked glycans of the Basidiomycetous yeasts Pseudozyma antarctica and Malassezia furfur (Subphylum Ustilaginomycotina).
J Gen Appl Microbiol., 2019 May 21;65(2):53-63.

Tsai YY, Ohashi T, Wu CC, Bataa D, Misaki R, Limtong S, Fujiyama K.
Delta-9 fatty acid desaturase overexpression enhanced lipid production and oleic acid content in Rhodosporidium toruloides for preferable yeast lipid production.
J Biosci Bioeng., 2019 Apr 127(4):430-440.

Ohashi H, Ohashi T, Misaki R, Fujiyama K.
Arabidopsis thaliana α1,2-l-fucosyltransferase catalyzes the transfer of l-galactose to xyloglucan oligosaccharides.
FEBS Lett., 2019 Jan 593(2):187-194.

Arofatullah NA, Kabirun S, Fujiyama K, Widianto D
Molecular identification and in vitro propagation of arbuscular mycorrhiza from tea plant rhizosphere
Current Research in Environmental & Applied Mycology 2019 9(1): 92–102
*本論文に示されている成果の一部は、Arofatullah NAがBiotechnology School in Asia programに在籍中になされてたものである

::学会発表 2019年度:

2018年

Fujiyama, K. (2018)
‘Glycoprotein Biosynthesis of Silkworm’, in Maenaka, K. and Park, E.Y. (eds.) Silkworm Biofactory. Boca Raton: CRC Press, pp. 107-126.

Nakamura M, Tran ML, Le QL, Suzuki H, Nguyen CM, Vu HG, Tamura K, Nguyen VT, Suzuki H, Misaki R, Muranaka T, Ninh KB, Fujiyama K, Seki H.
Transcriptome sequencing and identification of cytochrome P450 monooxygenases involved in the biosynthesis of maslinic acid and corosolic acid in Avicennia marina
Plant Biotechnology, 2018 Dec 25; 35(4): 341–348.

藤山和仁
カイコの高次な生物機能の“革命的”利用、研究ノート
生産と技術、第70巻、第4号、65-8 (2018)

Wu CC, Tsai YY, Ohashi T, Misaki R, Limtong S, Fujiyama K.
Isolation of a thermotolerant Rhodosporidium toruloides DMKU3-TK16 mutant and its fatty acid profile at high temperature.
FEMS Microbiol Lett., 2018 Nov 365(21).


Priyambada SA, Misaki R, Okamoto T, Ohashi T, Ueda K, Matsuura Y, Fujiyama K.
St6gal1 knockdown alters HBV life cycle in HepAD38 cells.
Biochem Biophys Res Commun., 2018 Sep 10; 503(3): 1841-184.


Naphatsamon U, Ohashi T, Misaki R, Fujiyama K.
The Production of Human β-Glucocerebrosidase in Nicotiana benthamiana Root Culture.
Int J Mol Sci., 2018 Jul 6; 19(7)

大橋貴生
機能性フラボノイド配糖体の醗酵生産、研究ノート
生産と技術、第70巻、第1号、103-5 (2018)

Suganuma M, Nomura T, Higa Y, Kataoka Y, Hunaguma S, Okazaki H, Suzuki T, Fujiyama K, Sezutsu H, Tatematsu K, Tamura T.
N-glycan sialylation in a silkworm-baculovirus expression system
J Biosci Bioeng., 2018 Jul 126(1): 9-14.

Nguyen TS, Misaki R, Ohashi T, Fujiyama K.
Enhancement of glycosylation by stable co-expression of two sialylation related enzymes on Chinese hamster ovary cells
J Biosci Bioeng., 2018 Jul 126(1): 102-110.

Morokuma D, Hino M, Masuda A, Mon H, Fujiyama K, Kajiura H, Kusakabe T, Lee JM,
A truncated form of human alpha 1-acid glycoprotein is useful as a molecular tool for insect glycobiology
Int J Indust. Entomol., 2018 36(1): 15-24.

Roychowdhury S, Youngjun Oh, Kajiura H, Hamorsky K, Fujiyama K, Matoba N.
Hydroponic treatment of Nicotiana benthamiana with kifunensine modifies the N-glycans of recombinant glycoprotein antigens to predominantly Man9 high-mannose type upon transient overexpression
Front. Plant Sci., 2018 Jan 30; 9: 62

Kajiura H,Hiwasa-Tanase K,Ezura H, Fujiyama K
Comparison of N-glycosylation on recombinant miraculin expressed in tomato plants with native miraculin
Plant Biotechnology, 2018 Vol. 35 No. 4 pp. 375-379.

Polburee P, Ohashi T, Tsai YY, Sumyai T, Lertwattanasakul N, Limtong S, Fujiyama K.
Molecular cloning and overexpression of DGA1, an acyl-CoAdependent diacylglycerol acyltransferase, in the oleaginous yeast Rhodosporidiobolus fluvialis DMKU-RK253
Microbiology, 2018 Jan 164(1): 1-10.

Ishinishi R, Matsuura H, Tanaka S, Nozawa S, Tanada K, Kawashita N, Fujiyama K, Miyasaka H, Hirata K.
Isolation and characterization of a stress-responsive gene encoding a CHRD domain-containing protein from a halotolerant green alga.
Gene, 2018 Jan 15; 640:14-20.

::学会発表 2018年度:

::2017年以前の発表論文::

Copyright (C) Fujiyama lab., ICBiotech, Osaka University