Fujiyama Lab. ICBiotech. Osaka University  
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Publications

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Publications in last 5years is Here

::Publications before 2017::

2017

Pierce OM, McNair GR, He X, Kajiura H, Fujiyama K, Kermode AR.
N-glycan structures and downstream mannose-phosphorylation of plant recombinant human alpha-L-iduronidase: toward development of enzyme replacement therapy for mucopolysaccharidosis I
Plant Mol Biol., 2017 Dec 95 (6): 593-606.

Ohashi H, Wahl C, Ohashi T, Elling L and Fujiyama K.
Effective synthesis of guanosine 5'-diphospho-β-L-galactose using bacterial L-fucokinase/guanosine 5'-diphosphate-L-fucose pyrophosphorylase
Advanced Synthesis & Catalysis, DOI: 10.1002/adsc.201700901

Priyambada SA, Misaki R, Okamoto T, Okamoto Y, Ohashi T, Ueda K, Matsuura Y, Fujiyama K.
Cell surface N-glycan alteration in HepAD38 cell lines expressing Hepatitis B virus.
Virus Res., 2017 Jun 20; 238:101-109.

Kajiura H, Suzuki N, Tokumoto Y, Yoshizawa T, Takeno S, Fujiyama K, Kaneko Y, Matsumura H, Nakazawa Y.
Two Eucommia farnesyl diphosphate synthases exhibit distinct enzymatic properties leading to end product preferences.
Biochimie, 2017 Aug 139:95-106.

Flores RJ, Ohashi T, Kawasaki H, Fujiyama K.
The Neutral N-linked Glycans of the Ustilaginomycete Yeast Sympodiomycopsis paphiopedili.
Yeast, 2017 Jul 34(7):305-317.

Ohashi T, Jinno J, Inoue Y, Ito S, Fujiyama K, Ishimizu T.
A polygalacturonase localized in the Golgi apparatus in Pisum sativum.
J Biochem., 2017 Sep 1;162(3):193-201.

  *Collaboration with Prof. Ishimizu of Ritsumeikan University

Uehara Y, Tamura S, Maki Y, Yagyu K, Mizoguchi T, Tamiaki H, Imai T, Ishii T, Ohashi T, Fujiyama K, Ishimizu T.
Biochemical characterization of rhamnosyltransferase involved in biosynthesis of pectic rhamnogalacturonan I in plant cell wall.
Biochem Biophys Res Commun, 2017 Apr 22;486(1):130-136.

  *Collaboration with Prof. Ishimizu of Ritsumeikan University

Ohashi H, Ohashi T, Kajiura H, Misaki R, Kitamura S, Fujiyama K.
Fucosyltransferases produce N-glycans containing core L-galactose.
Biochem Biophys Res Commun, 2017 Jan 29;483(1):658-663.

*Collaboration with Prof. Kitamura of Osaka Prefecture University

Tsai YY, Ohashi T, Kanazawa T, Polburee P, Misaki R, Limtong S, Fujiyama K.
Development of a sufficient and effective procedure for transformation of an oleaginous yeast, Rhodosporidium toruloides DMKU3-TK16.>
Curr Genet., 2017 May 63(2):359-371.

  *Collaboration with Kasetsert Univ (Thailand)

::Conference Presentations 2017

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2016

De Martinis D, Rybicki EP, Fujiyama K, Franconi R, Benvenuto E.
Editorial: Plant Molecular Farming: Fast, Scalable, Cheap, Sustainable.
Front Plant Sci, 2016 Aug 3; 7:1148

Misaki R, Fukura N, Kajiura H, Yasugi M, Kubota-Koketsu R, Sasaki T, Momota M,Ono K, Ohashi T, Ikuta K, Fujiyama K.
Recombinant production and characterization of human anti-influenza virus monoclonal antibodies identified from hybridomas fused with human lymphocytes.
Biologicals, 2016 44(5); 394-402.

Takamatsu S, Shimomura M, Kamada Y, Maeda H, Sobajima T, Hikita H, Iijima M, Okamoto Y, Misaki R, Fujiyama K, Nagamori S, Kanai Y, Takehara T, Ueda K, Kuroda S, Miyoshi E.
Core-fucosylation plays a pivotal role in hepatitis B pseudo virus infection: a possible implication for HBV glyco-therapy.
Glycobiology., 2016 Nov 26(11):1180-1189.

  *Collaboration with Graduate School of Medicine OU and the Institute of Scientific and Industrial Research OU
  *Supported by the Japan Agency for Medical Research and Development, AMED

Pirapan Polbureea P, Yongmanitchaia W, Honda K, Ohashi T, Yoshida T, Fujiyama K, Limtonga S
Lipid production from biodiesel-derived crude glycerol by Rhodosporidium fluviale DMKU-RK253 using temperature shift with high cell density
Biochem Eng J., 2016 112, 208-218.
   *Collaboration with Kasetsert Univ (Thailand)

Limkul J, Iizuka S, Sato Y, Misaki R, Ohashi T, Ohashi T, Fujiyama K.
The production of human glucocerebrosidase in glyco-engineered Nicotiana benthamiana plants.
Plant Biotechnol J., 2016 14(8); 1682-1694.

    *Collaboration with The Jikei University School of Medicine (Japan)

Trempel F, Kajiura H, Ranf S, Grimmer J, Westphal L, Zipfel C, Scheel D, Fujiyama K and Lee J
Altered glycosylation of exported proteins, including surface immune receptors, compromises calcium and downstream signaling responses to microbe-associated molecular patterns in Arabidopsis thaliana.
BMC Plant Biol., 2016 16(1): 31.

    *Collaboration with Leibniz Institute of Plant Biochemistry(Halle/Saale) (Germany)

Roongsawang N, Puseenam A, Kitikhun S, Sae-Tang K, Harnpicharnchai P, Ohashi T, Fujiyama K, Tirasophon W, Tanapongpipat S.
A Novel Potential Signal Peptide Sequence and Overexpression of ER-Resident Chaperones Enhance Heterologous Protein Secretion in Thermotolerant Methylotrophic Yeast Ogataea thermomethanolica.
Appl Microbiol Biotechnol., 2016 178(4); 710-724.

    *Collaboration with BIOTEC (Thailand)

::Conference Presentations 2016

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2015

Ohashi T, Hasegawa Y, Misaki R, Fujiyama K.
Substrate preference of citrus naringenin rhamnosyltransferases and their application to flavonoid glycoside production in fission yeast.
Appl Microbiol Biotechnol., 2015 100(2):687-696.

Polburee P, Yongmanitchai W, Lertwattanasakul N, Ohashi T, Fujiyama K, Limtong S.
Characterization of oleaginous yeasts accumulating high levels of lipid when cultivated in glycerol and their potential for lipid production from biodiesel-derived crude glycerol
Fungal Biol., 2015 119(12):1194-1204.

 *Collaboration with Kasetsert Univ (Thailand)

Limkul J, Misaki R, Kato K, Fujiyama K.
The combination of plant translational enhancers and terminator increase the expression of human glucocerebrosidase in Nicotiana benthamiana plants
Plant Science, 2015 240: 41-49.

Kajiura H, Hamaguchi Y, Mizushima H, Misaki R, Fujiyama K.
Sialylation potentials of the silkworm, Bombyx mori; B. mori possesses an active α2,6-sialyltransferase.
Glycobiology, 2015 25(12): 1441-1453.

Arief II, Jenie BSL, Astawan M, Fujiyama K, Witarto AB.
Identification and probiotic characteristics of lactic acid bacteria isolated from Indonesian local beef
Asian J Animal Sciences, 2015 9(1): 25-36.

  *Collaboration with IPB (Indonesia)

H. Kajiura, K. Fujiyama
Im“plant”ing of Mammalian Glycosyltransferase Gene into Plant Suspension-Cultured Cells Using Agrobacterium-Mediated Transformation
Methods Mol Biol., 2015 1321:225-232.

Hamorsky KT, Kouokam JC, Jurkiewicz JM, Nelson B, Moore LJ, Husk AS, Kajiura H, Fujiyama K, Matoba N.
N-glycosylation of cholera toxin B subunit in Nicotiana benthamiana: impacts on host stress response, production yield and vaccine potential.
Sci Rep., 2015 5:8003.

Srichaisupakit A, Ohashi T, Misaki R, Fujiyama K.
Production of the Initial-Stage Eukaryotic N-Glycan and Protein Glycosylation in Escherichia coli.
J Biosci Bioeng., 2015 119(4): 399-405.

Nomura T, Suganuma M, Higa Y, Kataoka Y, Funaguma S, Okazaki H, Suzuki T, Kobayashi I, Sezutsu H, Fujiyama K.
Improvement of glycosylation structure by suppression of β-N-acetylglucosaminidases in silkworm.
J Biosci Bioeng., 2015 119(2): 131-136.

::Conference Presentations 2015

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2014

Fujiyama K.
Heterologous Glycoprotein Production (Plant)
In: Glycoscience: Biology and Medicine. Naoyuki Taniguchi,Tamao Endo, Gerald W. Hart, Peter H. Seeberger, Chi-Huey Wong (Ed.) Springer Pages 1545-1549 ISBN: 978-4-431-54840-9 (Print) 978-4-431-54841-6 (Online)., 2014 Mar

Polburee P, Yongmanitchai W, Ohashi T, Fujiyama K, Limtong S.
Barnettozyma siamensis f.a., sp. nov., a novel lipid accumulating ascomycete yeast species isolated in Thailand.
Int J Syst Evol Microbiol., 2014 64(Pt 9): 3053-3057.

Yumioka-Ito H, Misaki R, Yokoro M, Suzuki M, Yamashita H, Hiemori-Kondo M, Kimoto M, Kato K, Fujiyama K, Tsuji H.
Cloning of a cDNA encoding the Gly m Bd 28K precursor and its vacuole transport in tobacco BY2 suspension-cultured cells.
J Nutr Sci Vitaminol., 2014 60(2): 129-139.

Pitaksajjakul P, Benjathummarak S, Pipattanaboon C, Wongwit W, Okabayashi T, Kuhara M, Misaki R, Fujiyama K, Ramasoota P.
Antibody Germline Characterization of Cross-neutralizing human IgGs against 4 serotypes of Dengue virus.
Biochem Biophys Res Commun., 2014 446(2): 475-80.

Srichaisupakit A, Ohashi T, Fujiyama K.
Identification of a protein glycosylation operon from Campylobacter jejuni JCM 2013 and its heterologous expression in Escherichia coli.
J Biosci Bioeng., 2014 118(3): 256-262.

::Conference Presentations 2014

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2013

Yasugi M, Kubota-Koketsu R, Yamashita A, Kawashita N, Du A, Misaki R, Kuhara M, Boonsathorn N, Fujiyama K, Okuno Y, Nakaya T, Ikuta K.
Emerging Antigenic Variants at the Antigenic Site Sb in Pandemic A(H1N1)2009 Influenza Virus in Japan Detected by a Human Monoclonal Antibody.
PLoS One., 2013 8(10): e77892

Kajiura H, Wasai M, Kasahara S, Takaiwa F, Fujiyama K.
N-Glycosylation and N-glycan moieties of CTB expressed in rice seeds.
Mol Biotechnol., 2013 54(3): 784-794.

Wang S, Takahashi H, Kajiura H, Kawakatsu T, Fujiyama K, Takaiwa F.
Transgenic Rice Seeds Accumulating Recombinant Hypoallergenic Birch Pollen Allergen Bet v 1 Generate Giant Protein Bodies.
Plant Cell Physiol., 2013 54(6): 917-933.

Sasaki T, Setthapramote C, Kurosu T, Nishimura M, Asai A, Omokoko MD, Pipattanaboon C, Pitaksajjakul P, Limkittikul K, Subchareon A, Chaichana P, Okabayashi T, Hirai I, Leuangwutiwong P, Misaki R, Fujiyama K, Ono K, Okuno Y, Ramasoota P, Ikuta K.
Dengue virus neutralization and antibody-dependent enhancement activities of human monoclonal antibodies derived from dengue patients at acute phase of secondary infection.
Antiviral Res., 2013 98(3): 423-431.

Hamorsky KT, Kouokam JC, Bennett LJ, Baldauf KJ, Kajiura H, Fujiyama K, Matoba N.
Rapid and scalable plant-based production of a cholera toxin B subunit variant to aid in mass vaccination against cholera outbreaks.
PLoS Negl Trop Dis., 2013 7(3): e2046

Yasugi M, Kubota-Koketsu R, Kawashita N, Anariwa D, Misaki R, Kuhara M, Boonsathorn N, Fujiyama K, Okuno S, Nakaya T, Ikuta K.
Human Monoclonal Antibodies Broadly Neutralizing against Influenza B Virus.
PLoS Pathog., 2013 9(2): e1003150

Watanabe S, Kakudo A, Ohta M, Mita K, Fujiyama K, Inumaru S.
Molecular cloning and characterization of the alpha-glucosidase II from Bombyx mori and Spodoptera frugiperda.
Insect Biochem Mol Biol., 2013 43(4): 319-327.

::Conference Presentations 2013

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2012

Kajiura H, Tanaka R, Kato K, Hamaguchi Y, Seki T, Fujiyama K.
N-Glycan Structures of glycoproteins in suspension-cultured Arabidopsis thaliana T87 cells.
Plant Biotechnol., 2012 29: 489-493.

Kajiura H, Wasai M, Kasahara S, Takaiwa F, Fujiyama K.
N-Glycosylation and N-glycan moieties of CTB expressed in rice seeds. Mol Biotechnol
., 2012 54(3): 784-794.

Ohashi T, Fujiyama K, Takegawa K.
Identification of novel α1,3-galactosyltransferase and elimination of
α-galactose-containing glycans by disruption of multiple
α-galactosyltransferase genes in Schizosaccharomyces pombe.
J Biol Chem., 2012 287(46): 38866-75.

Hong Z, Kajiura H, Su W, Jin H, Kimura A, Fujiyama K, Li J.
Evolutionarily conserved glycan signal to degrade aberrant brassinosteroid receptors in Arabidopsis.
Proc Natl Acad Sci USA., 2012 109(28):11437-42.

Lombardi R, Donini M, Villani ME, Brunetti P, Fujiyama K, Kajiura H, Paul M, Ma J, Benvenuto E.
Production of different glycosylation variants of the tumour-targeting mAb H10 in Nicotiana benthamiana. Influence on expression yield and antibody degradation.
Transgenic Res., 2012 21(5): 1005-21.

Onitsuka M, Kim WD, Ozaki H, Kawaguchi A, Honda K, Kajiura H, Fujiyama K, Asano R, Kumagai I, Ohtake H, Omasa T.
Enhancement of sialylation on humanized IgG-like bispecific antibody by overexpression of α2,6-sialyltransferase derived from Chinese hamster ovary cells.
Appl Microbiol Biotechnol., 2012 94(1): 69-80.

Kajiura H, Okamoto T, Misaki R, Matsuura Y, Fujiyama K.
Arabidopsis β1,2-xylosyltransferase: Substrate specificity and participation in the plant-specific N-glycosylation pathway.
J Biosci Bioeng., 2012 113(1): 48-54.

::Conference Presentations 2012

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2011

R. Misaki, Y. Sakai, T. Omasa, K. Fujiyama, and T. Seki.
N-terminal vacuolar sorting signal at the mouse antibody alters the N-linked glycosylation pattern in suspension-cultured tobacco BY2 cells.
J Biosci Bioeng., 2011 112(5): 476-484.

Y. Kimura, Y. Takeoka, M. Inoue, M. Maeda, and K. Fujiyama.
Double-Knockout of Putative Endo-β-N-acetylglucosaminidase (ENGase) Genes in Arabidopsis thaliana: Loss of ENGase Activity Induced Accumulation of High-Mannose Type Free N-Glycans Bearing N,N'-Acetylchitobiosyl Unit.
Biosci Biotechnol Biochem., 2011 75(5): 1019-21.

H. Kaulfuerst-Soboll, S. Rips, H. Koiwa, H. Kajiura, K. Fujiyama, and A. von Schaewen.
Reduced immunogenicity of Arabidopsis hybrid glycosylation1 (hgl1) N-glycans due to altered accessibility of xylose and core fucose epitopes.
J Biol Chem., 2011 286(26): 22955-64.

J. Isoyama-Tanaka, K. Dohi, R. Misaki, and K. Fujiyama.
Improved expression and characterization of recombinant human Golgi α1,2-mannosidase I isoforms (IA2 and IC) by Escherichia coli.
J Biosci Bioeng.,2011 112(1): 14-9.

K. Dohi, J. Isoyama-Tanaka, R. Misaki, and K. Fujiyama.
Jack bean alpha-mannosidase digestion profile of hybrid-type N-glycans: effect of reaction pH on substrate preference.
Biochimie., 2011 93(4): 766-71.

H. Kajiura, R. Misaki, K. Fujiyama, and T. Seki.
Stable coexpression of two human sialylation enzymes in plant suspension-cultured tobacco cells.
J Biosci Bioeng., 2011 111(4): 471-7.

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2010

R. Misaki, M. Morimatsu, T. Uemura, S. Waguri, E. Miyoshi, N. Taniguchi, M. Matsuda, and T. Taguchi.
Palmitoylated Ras proteins traffic through recycling endosomes to the plasma membrane during exocytosis.
J Cell Biol., 2010 191(1): 23-9.

T. Nomura, M. Ikeda, S. Ishiyama, K. Mita, T. Tamura, T. Okada, K. Fujiyama, and A. Usami.
Cloning and characterization of a beta-N-acetylglucosaminidase (BmFDL) from silkworm Bombyx mori.
J Biosci Bioeng., 2010 110(4): 386-91.

H. Kajiura, T. Seki, and K. Fujiyama.
Arabidopsis thaliana ALG3 mutant synthesizes immature oligosaccharides in the ER and accumulates unique N-glycans.
Glycobiology., 2010 20(6): 736-751.

K. Dohi, J. Isoyama-Tanaka, T. Tokuda, and K. Fujiyama
Recombinant Expression and Characterization of N-Acetylglucosaminyltransferase I Derived from Nicotiana tabacum.
J Biosci Bioeng., 2010 109 (4): 388-391.

MFM. Khalil, H. Kajiura, K. Fujiyama, K. Koike, N. Ishida, and N. Tanaka
Tobacco plants overexpressing human UDP-galactose transporter gene hUGT1 display enhanced growth and the gibberellin-responsive phenotype
J. Biosci. Bioeng., 2010 109(2): 159-169.

H. Kajiura, H. Koiwa, Y. Nakazawa, A. Okazawa, A. Kobayashi, T. Seki, and K. Fujiyama.
Two Arabidopsis thaliana Golgi {alpha}-mannosidase Is are responsible for plant N-glycan maturation.
Glycobiology., 2010 20(2): 235-247.

R. Kaewwichian, W. Yongmanitchai, N. Srisuk, K. Fujiyama, and S. Limtong.
Geotrichum siamensis sp. nov. and Geotrichum phurueaensis sp. nov., two asexual arthroconidial yeast species isolated in Thailand.
FEMS Yeast Res., 2010 10: 214-220.

WD. Kim, M. Tokunaga, H. Ozaki, T. Ishibashi, K. Honda, H. Kajiura, K. Fujiyama, R. Asano, I. Kumagai, T. Omasa, and H. Ohtake.
Glycosylation pattern of humanized IgG-like bispecific antibody produced by recombinant CHO cells.
Appl Microbiol Biotechnol., 2010 85(3): 535-542.

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2009

S. Takashima, J. Seino, T. Nakano, K. Fujiyama, M. Tsujimoto, N. Ishida, and Y. Hashimoto
Analysis of CMP-sialic acid transporter-like proteins in plants.
Phytochemistry, 2009 70(17-18), 1973-1981.

R. Misaki, H. Kajiura, K. Fujii, K. Fujiyama, and T. Seki
Cloning and Characterization of Cytidine Monophosphate-3-Deoxy-D-Manno-Octulosonate Synthetase from Arabidopsis thaliana.
J. Biosci. Bioeng. 2009 108(6), 527-529.

K. Nakamura, M. Inoue, M. Maeda, R. Nakano, K. Hosoi, K. Fujiyama, and Y. Kimura.
Molecular cloning and gene expression analysis of tomato endo-beta-N-acetylglucosaminidase, an endoglycosidase involved in the production of high-mannose type free N-glycans during tomato fruit ripening.
Biosci Biotechnol Biochem., 2009 73(2), 461-464.

S. Limtong, W. Yongmanitchai, H. Kawasaki, and K. Fujiyama.
Wickerhamomyces edaphicus sp. nov. and Pichia jaroonii sp. nov., two ascomycetous yeast species isolated from forest soil in Thailand.
FEMS Yeast Res., 2009 9, 504-510.

N. Matoba, H.Kajiura, I. Cherni, J. D Doran, M. Bomsel, K. Fujiyama and T. S Mor.
Biochemical and Immunological Characterization of the plant-derived candidate HIV-1 mucosal Vaccine CTB MPR 649-684.
Plant Biotechnol J., 2009 7, 129-145.
See also the front cover page.

K. Fujiyama, Y. Sakai, R. Misaki,T. Omasa, and T. Seki.
Change in glycosylation pattern with extension of endoplasmic reticulum retention signal sequence of mouse antibody produced by suspension-cultured tobacco BY2 cells.
J. Biosci. Bioeng., 2009 107, 165-172.

M. Kilcoyne, M. Shah, J. Q Gerlach, V. Bhavanandan, V. Nagaraj, A. D Smith, K. Fujiyama, U. Sommer, C. E Costello, N. Olszewski, and L. Joshi.
O-glycosylation of protein subpopulations in alcohol-extracted rice proteins.
J Plant Physiol., 2009 166(3), 219-232.

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2008

J.S. Kang, J. Frank, C.H. Kang, H. Kajiura, M. Vikram, A. Ueda, S. Kim, J.D. Bahk, B. Triplett, K. Fujiyama, S.Y. Lee, A. von Schaewen, and H. Koiwa.
Salt tolerance of Arabidopsis thaliana requires maturation of N-glycosylated proteins in the Golgi apparatus.
Proc, Natl, Acad, Sci, USA, 2008 105(15), 5933-5938.
   Erratum in Proc. Natl. Acad. Sci. USA, 2008 105(22), 7893

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2007

Y. Kimura, T. Watanabe, M. Maeda, Y. Murata, and K. Fujiyama.
Salt-adaptation of Tobacco BY2 Cells Induces Change in Glycoform of N-Glycans: Enhancement of Exo- and Endo-glycosidase Activities by Salt-adaptation
Biosci. Biotechnol. Biochem., 2007 71 (4), 1111-1114.

L. Yang, H. Kajiura, K. Suzuki, S. Hirose, K. Fujiyama, and F. Takaiwa.
Generation of a transgenic rice seed-based edible vaccine against house dust mite allergy.
Biochem. Biophys. Res. Commun., 2007 365, 334-339.

W. Saejung, K. Fujiyama, T. Takasaki, M. Ito, K. Hori, P. Malasit, Y. Watanabe, I. Kurane and T. Seki.
Production of dengue 2 envelope domain III in plant using TMV-based vector system.
Vaccine, 2007 25 (36), 6646-6654.

H. Tani, Y. Komoda, E. Matsuo, K. Suzuki, I. Hamamoto, T. Yamashita, K. Moriishi, K. Fujiyama, T. Kanto, N. Hayashi, A. Owsianka, A. H. Patel, M. A. Whitt, and Y. Matsuura.
Replication-Competent Recombinant Vesicular Stomatitis Virus Encoding Hepatitis C Virus Envelope Proteins.
J. Virology, 2007 81(16), 8601-8612.

Y.Kimura, S. Sakamura, T. Ushijima, Y. Hama, H. Kajiura, K. Fujiyama, K. Okihara, K. Hashimoto, H. Sugimoto, and H. Yamada.
First evidence for b1-3 galactosyltransferase activity involved in biosynthesis of insect complex type N-glycan harboring T-antigen in Apis mellifera.
Biosci. Biotechnol. Biochem., 2007 71(4), 1111-1114.

K. Fujiyama, A. Furukawa, A. Katsura, R. Misaki, T. Omasa and Seki.
Production of mouse monoclonal antibody with galactose-extended sugar chain by suspension cultured tobacco BY2 cells expressing human b(1,4)-galactosyltransferase.
Biochem. Biophys. Res. Commun., 2007 358(1), 85-91.

T. Okada, S, Ishiyama, H. Sezutsu, A. Usami,T. Tamura, K. Mita, K. Fujiyama, and T. Seki.
Molecular cloning and expression of two novel b-N-acetylglucosaminidases from Silkworm Bombyx mori .
Biosci. Biotechnol. Biochem., 2007 71(7), 1626-1635.

K. Fujiyama, R. Tanaka, M. Terashima, and T. Seki.
N-Linked glycan structures of glycoproteins in suspension-cultured Arabidopsis thaliana MM2d cells.
Plant Biotechnology, 2007 24, 255-259.

M.N. Cahyanto, H. Kawasaki, M. Nagashio, K. Fujiyama and T. Seki.
Construction of Lactobacillus plantarum strain with enhanced L-lysine yield.
J. Appl. Microbiol., 2007 102, 674-679.

Japanese Only
藤山和仁

「植物細胞による組換えタンパク質生産:培養細胞は閉鎖型バイオリアクター」
化学と生物 2007年 Vo. 45, No. 7, 458-460.

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2006

W. Saejung, C. Puttikhunt, T. Prommool, P. Sojikul, R. Tanaka, K. Fujiyama, P. Malasit, and T. Seki.
Enhancement of recombinant soluble dengue virus 2 envelope domain III protein production in Escherichia coli trxB and gor double mutant.
J. Biosci. Bioeng., 2006 102, 333-339.

K. Fujiyama, W. Saejung, I. Yanagihara, J. Nakado, R. Misaki, T. Honda, Y. Watanabe, and T. Seki.
In planta production of the immunogenic poliovirus peptide using TMV-based vector system.
J. Biosci. Bioeng., 2006 101, 398-402.

M. N. Cahyanto, H. Kawasaki, M. Nagashio, K. Fujiyama and T. Seki.
Regulation of aspartokinase, aspartate semialdehyde dehydrogenase, dihydrodipicolinate synthase, and dihydrodipicolinate reductase in Lactobacillus plantarum.
Microbiology, 2006 152, 105-112.

R. Misaki, K. Fujiyama and T. Seki.
Expression of human CMP-N-acetylneuraminic acid synthetase, and CMP-sialic acid transporter in tobacco suspension-cultured cell.
Biochem. Biophys. Res. Commun., 2006 339, 1184-1189.

K. Fujiyama, R. Misaki, A. Katsura, T. Tanaka, A. Furukawa, T. Omasa and T. Seki.
N-Linked glycan structures of a mouse monoclonal antibody produced from tobacco BY2 suspension-cultured cells.
J. Biosci. Bioeng., 2006 101, 212-218.

Japanese Only
藤山和仁

「組換え医療用糖タンパク質の生産に向けた宿主細胞の育種」
酵素工学ニュース 2006年 第55号 17-23.

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2005

M. Nur Cahyanto, H. Kawasaki, K. Fujiyama and T. Seki.
Cloning of IAM 12477 lysine biosynthetic genes encoding functional aspartate semialdehyde dehydrogenase, dihydrodipicolinate synthase, and dihydrodipicolinate reductase.
World J. of Microbiol. Biotechnol., 2006 22, 409-416.

S. Nandi, D. Yalda, S. Lu, Z. Nikolov, R. Misaki, K. Fujiyama and N. Huang.
Process development and economic evaluation of recombinant human lactoferrin expressed in rice grain.
Transgenic Research, 2005 14, 237-249.

Japanese Only
藤山和仁

「植物バイオリアクターによる外来タンパク質生産 -特集によせて-」
生物工学会誌 第83号 第11号 509 (2006)
「植物生産医療タンパク質と糖鎖」
生物工学会誌 第83号 第11号 522-524 (2006)

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2004

K. Fujiyama, Y. Sakai, R. Misaki, I. Yanagihara, T. Honda, H. Anzai and T. Seki.
N-linked glycan structures of human lactoferrin produced by rice.
Biosci. Biotechnol. Biochem., 2004 68, 2565-2570.

Japanese Only
藤山和仁

「分子農業を目指した植物糖鎖工学」
バイオサイエンスとバイオインダストリー Vo. 62, No. 12, 19-24 (2004)

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2003

R, Misaki. Y, Kimura. N. Q. Palacpac, S, Yoshida. K, Fujiyama. T. Seki.
Plant cultured cells expressing human beta1,4-galactosyltransferase secrete glycoproteins with galactose-extended N-linked glycans.
Glycobiology, 2003 13, 199-205.

R. Misaki, K. Fujiyama, H. Yokoyama, Y. Ido, K. Miyauchi, T. Yoshida, and T. Seki.
Characterization of almond a-mannosidase and its application for structureanalysis of sugar chain.
J. Biosci. Bioeng., 2003 96, 187-192.

R. Misaki, H. Nagaya, K. Fujiyama, I, Yanagihara, T. Honda, and T. Seki.
N-linked glycan structure of mouse interferon-b produced by Bombyx mori larvae.
Biochem. Biophys. Res. Commun., 2003 311, 979-986.

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2001

K. Fujiyama, Y. Ido, R. Misaki, D. G. Moran, I. Yanagihara, T. Honda, S. Nishimura, T. Yoshida and T. Seki.
Human N-acetylglucosaminyltransferase I. Expression in E. coli as a soluble enzyme, and application as an immobilized. enzyme for the chemoenzymatic synthesis of N-linked oligosaccharides.
J. Biosci. Bioeng., 2001 92, 569-574.

K. Fujiyama, Y. Kira, M. Iizuka, Y. Kimura and T. Seki.
Identification of putative gene encoded on ORF16 of the 81 kb contig of Arabidopsis thaliana chromosome III as a-mannosidase.
J. Biosci. Bioeng., 2001 92, 401-404.

R. Misaki, Y. Kimura, K. Fujiyama and T. Seki.
Glycoproteins Secreted from Suspension-cultured Tobacco BY2 Cells have Distinct Glycan Structures from Intracellular Glycoproteins.
Biosci. Biotechnol. Biochem., 2001 65, 2482-2488.

K. Fujiyama, S. Sakuradani, D. G. Moran, T. Yoshida and T. Seki.
Effect of a-1,2-mannosidic linkage located in a1,3-branch of Man6GlcNAc2 oligosaccharide on enzyme activity of recombinant human Man9-mannosidase produced in Escherichia coli.
J. Biosci. Bioeng., 2001 91, 419-421.

S. Shibatani, K. Fujiyama, S. Nishiguchi, T. Seki and Y. Maekawa.
Production and characterization of an active and soluble human b1,4-galactosyltransferase in Escherichia coli as a useful catalyst in the synthesis of Galb1-->4 GlcNAc linkage.
J. Biosci. Bioeng., 2001 91, 85-87.

K. Fujiyama, N. Q. Palacpac, H, Sakai. Y, Kimura. A, Shinmyo. T, Yoshida. T. Seki
In vivo conversion of a glycan to human compatible type by transformed tobacco cells.
Biochem Biophys Res Commun., 2001 289, 553-557.

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2000

W. Panbangred, K. Weeradechapon, S. Udomvaraphant, K. Fujiyama and V. Meevootisom.
High expression of penicillin G acylase gene (pac) from Bacillus megaterium UN1 in its pac minus mutant.
J. Appl. Microbiol., 2000 89 , 152-157.

1999

T. Okuda, A. G. Pue, K. Fujiyama, and K. Yokotsuka.
Purification and characterization of polyphenol oxidase from muscat bailey A grape juice.
American J. Enol. Viticul., 1999 50, 137-143.

N. Q. Palacpac, S. Yoshida, H. Sakai, Y. Kimura, K. Fujiyama, T. Yoshida and T. Seki.
Stable expression of human b1,4-galactsosyltransferase gene in plant cells modifies N-glycosylation.
Proc. Natl. Acad. Sci. USA, 1999 96, 4692-4697.

N. Q. Palacpac, Y. Kimura, K. Fujiyama, T. Yoshida and T. Seki.
Structures of N-linked oligosaccharides of glycoproteins from tobacco BY2 suspension cultured cells.
Biosci. Biotechnol. Biochem., 1999 63, 35-39.

1998

S. Teeradakorn, Y. Talawanich, I. Suzuki, P. Pinphanichakarn, K. Fujiyama,T. Seki, and T. Yoshida.
Characterization of Streptomyces D3 derived from protoplast fusion between Streptomyces cyaneus 190-1 and Streptomyces. griseoruber 42-9.
World J. Microbiol. Biotechnol., 1998 14, 565-570.

D. G. Moran, S. Sakuradani, K. Fujiyama, T. Seki and T. Yoshida.
Characterization of recombinant human Man9-mannosidase expressed in Escherichia coli.
J. Ferment. Bioeng., 1998 86, 277-283.

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Copyright (C) Fujiyama lab., ICBiotech, Osaka University